Protein Info for BPHYT_RS06295 in Burkholderia phytofirmans PsJN

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR00229: PAS domain S-box protein" amino acids 13 to 135 (123 residues), 39.4 bits, see alignment E=6.2e-14 PF00989: PAS" amino acids 13 to 109 (97 residues), 35.8 bits, see alignment E=1.8e-12 PF08448: PAS_4" amino acids 17 to 106 (90 residues), 23.4 bits, see alignment E=1.5e-08 PF13426: PAS_9" amino acids 20 to 126 (107 residues), 34.6 bits, see alignment E=4.8e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 138 to 295 (158 residues), 68.8 bits, see alignment E=4.7e-23 PF00990: GGDEF" amino acids 139 to 289 (151 residues), 89.8 bits, see alignment E=4.2e-29 PF00563: EAL" amino acids 315 to 549 (235 residues), 255.3 bits, see alignment E=1.3e-79

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1278)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T282 at UniProt or InterPro

Protein Sequence (565 amino acids)

>BPHYT_RS06295 diguanylate phosphodiesterase (Burkholderia phytofirmans PsJN)
MQVPLYAETRTRDAALASAPYGMFTCTADGTFDCVNAAFEKLTGLAAEELIGRRTFESLF
DPAELAKRRAELPSLAMVTSRYEGEWSFLRRNGTPVRVVLALAPLVTEPARTACPALYVG
IAIDMTRYAQSEARLWYVAHHDGVTRLPNQTLFTERLELTIARCERSGSGFTVLIAELDH
LRKLRDALGLHAAELVLQIVGERLRGLFPNDGTIASIGGTQFALLVNETGPAADAFAAEA
LGRIAEAIDYAGTALNITASIGIVAYPADGGDAPTLMRRAGVALSAASAVNGNAVRRFST
ALEGQAARRFELQTMLRDAIDRQQLHLVYQPQVTLSNGHIAQVEALLRWNHPQRGLISPV
EFVPVAEEAGLIEQIGEWVIRTACRDAGKLLRLTGNLPRFAVNVSPQQFQRHNLFETIRE
ALEDAALDPSYLEVEITEGVLLGDTEQALQTLFALRDLGVEVAVDDFGTGYSSLAYLTRF
PLNRLKIDRSFVMRMSTDPQCAALVGAIIAMAHALKLRVTAEGVETAEQAAQLEALGCDE
AQGFWFSRPITVAALRNLLRPLGTL