Protein Info for BPHYT_RS06170 in Burkholderia phytofirmans PsJN

Annotation: DNA polymerase III subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1192 TIGR00594: DNA polymerase III, alpha subunit" amino acids 9 to 1025 (1017 residues), 1199.3 bits, see alignment E=0 PF02811: PHP" amino acids 11 to 177 (167 residues), 148.4 bits, see alignment E=7.7e-47 PF07733: DNA_pol3_alpha" amino acids 296 to 565 (270 residues), 345.7 bits, see alignment E=5.9e-107 PF17657: DNA_pol3_finger" amino acids 568 to 733 (166 residues), 214.3 bits, see alignment E=2.1e-67 PF14579: HHH_6" amino acids 807 to 901 (95 residues), 104.5 bits, see alignment E=8.5e-34 PF01336: tRNA_anti-codon" amino acids 991 to 1064 (74 residues), 36.3 bits, see alignment 1.3e-12 PF20914: DNA_pol_IIIA_C" amino acids 1148 to 1180 (33 residues), 33.5 bits, see alignment (E = 9e-12)

Best Hits

Swiss-Prot: 55% identical to DPO3A_NEIMB: DNA polymerase III subunit alpha (dnaE) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K02337, DNA polymerase III subunit alpha [EC: 2.7.7.7] (inferred from 87% identity to bcn:Bcen_1701)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T257 at UniProt or InterPro

Protein Sequence (1192 amino acids)

>BPHYT_RS06170 DNA polymerase III subunit alpha (Burkholderia phytofirmans PsJN)
MSPMSDPRFVHLRVHSEFSIADGIVRLDDIVKAAAKDGQGALALTDLGNAFGLVRFYKEA
RGKGVKPIAGCDVWITNPDDRDKPSRLLLLVKDRRGYLNLCELLSKASLTNQYRGRAEVE
AGWLESGLGEGLLALSGAQQGDVGLALAAGNEEAAKRNALHWAKVFPGGFYIELQRSGQP
GGEQYVQQAVALAASLKLPVVATHPMQFMTPDDFTAHEARVCISEGDILANPRRSKRFTS
EQYFRTQEEMAALFADIPSALANSVEIAKRCNLTLELGKPKLPLFPTPDGMSLDDYLVQL
SKEGLEKRLEQLYPDAAEREAQRETYYARLEFECGTIIKMGFPGYFLIVADFINWAKNNG
VPVGPGRGSGAGSLVAYALGVTDLDPLRYNLLFERFLNPERVSMPDFDIDFCQHGRDRVI
QYVKEKYGADAVSQIATFGTMAAKAAVRDIGRVLDLGYMFTDGIAKLIPFKPGKHVTIAD
AMKEEPLLQERFDNEDEVHQLLELAQRVEGLTRNVGMHAGGVLIAPGKLTDFCPLYTQGD
ESGVVSQYDKDDVEAVGLVKFDFLGLTTLTILDWAERYIRRLDPSKQDWSLGQVPLDDPA
SFSILKKANTVAVFQLESRGMQGMLKDAQPDRFEDIIALVALYRPGPMDLIPSFCARKHG
REVVEYPDPRVESVLRETYGIMVYQEQVMQMAQIIGGYSLGGADLLRRAMGKKKAEEMAE
HRELFRQGAAKNGLTAEKADEIFDLMEKFAGYGFNKSHAAAYALLAYYTAWLKAHHPAEF
MAANMSLAMDDTDKVKILFEDCLANKMAVLPPDVNLSAYRFEPVAEPDGKRSKTIRYGLG
AIKGSGQNAIEEILRAREEGPFIDIFDFCNRVDRRIVNRRTVEALIRAGAFDTLHANRAQ
LIASVSLAMEAAEQASANALQAGLFDMGDAPSQGHELVDEPEWPEKKKLQEEKAALGFYL
SGHLFDAYKNEVRRFVRQKIGELKEGRDKLVAGVIASLRTQMTQRGKMLIALLDDGTGQC
EVTVFNETFEAHKQLFKEDELLVVQGQARNDAFTGGIRFTVDTVMDLGRARCRYAEAVKV
QMNGNADALALRRVLEAHSAGKDEPQAVAAPAASSRGGNGGNGGGRNGGGYGGGGQRQAV
QIPNGLAVQVVYRSQNAEGEMRLGDAWRVKPTDELLAALRGEFAGSSIEIVY