Protein Info for BPHYT_RS06160 in Burkholderia phytofirmans PsJN

Annotation: glutamyl-Q tRNA(Asp) ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 TIGR03838: glutamyl-queuosine tRNA(Asp) synthetase" amino acids 3 to 276 (274 residues), 396.8 bits, see alignment E=2.5e-123 PF00749: tRNA-synt_1c" amino acids 4 to 248 (245 residues), 143.7 bits, see alignment E=3.2e-46

Best Hits

Swiss-Prot: 79% identical to GLUQ_BURCH: Glutamyl-Q tRNA(Asp) synthetase (gluQ) from Burkholderia cenocepacia (strain HI2424)

KEGG orthology group: K01894, glutamyl-Q tRNA(Asp) synthetase [EC: 6.1.1.-] (inferred from 100% identity to bpy:Bphyt_1251)

Predicted SEED Role

"glutamyl-Q-tRNA synthetase" in subsystem Queuosine-Archaeosine Biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.-

Use Curated BLAST to search for 6.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T255 at UniProt or InterPro

Protein Sequence (296 amino acids)

>BPHYT_RS06160 glutamyl-Q tRNA(Asp) ligase (Burkholderia phytofirmans PsJN)
MTYRGRFAPSPTGPLHFGSLVSALASWLDARAHRGAWLVRVEDIDGPRTVPGAAEDILAT
LERFGMHADEPPVWQSARMPRYQEALEQLKANGVIYPCGCTRKEIADSLLNAHTRNTTLA
YPGTCRDGLHGKPARAWRLRVPDGDSAVITFEDRWQGKQTQNLATEVGDFVLKRADDQWA
YQLAVVVDDADAGITHIVRGADLMDSTARQIYLQRCLGVPTPEYLHVPVVTNEAGEKLSK
QNGATALDSEKPFEALSAAARHLGLDLENAAAQTTLDGFYAAATAAWAKRMGLPAA