Protein Info for BPHYT_RS06155 in Burkholderia phytofirmans PsJN

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 PF00270: DEAD" amino acids 35 to 208 (174 residues), 168.2 bits, see alignment E=2e-53 PF04851: ResIII" amino acids 38 to 202 (165 residues), 34.5 bits, see alignment E=2.9e-12 PF00271: Helicase_C" amino acids 244 to 352 (109 residues), 102.9 bits, see alignment E=1.8e-33

Best Hits

Swiss-Prot: 39% identical to RHLB_SHEFN: ATP-dependent RNA helicase RhlB (rhlB) from Shewanella frigidimarina (strain NCIMB 400)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1250)

Predicted SEED Role

"ATP-dependent RNA helicase Bcep18194_A5658" in subsystem ATP-dependent RNA helicases, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T254 at UniProt or InterPro

Protein Sequence (489 amino acids)

>BPHYT_RS06155 DEAD/DEAH box helicase (Burkholderia phytofirmans PsJN)
MSDTAVTPSTATFDQFGLAPDILKAVKESGYTIPTPIQEQAIPVVLAGRDMMGAAQTGTG
KTASFSLPIIQRLLPQASTSASPARHPVRALILTPTRELADQVAANVQAYAKHTALRSAV
VFGGVDMNPQSEQLRRGVEILIATPGRLLDHVQQKTANLGQVQILVLDEADRMLDMGFLP
DLQRILNLLPKERQTLLFSATFSGEIKKLAATYLRNPQTIEVARSNSTATNVTQVVYEVA
EGDKTGAVVQLIRERGLKQVIVFCNSKIGASRLARSLERDGVVATAIHGDRTQNERMQAL
DAFKRGEIEALVATDVAARGLDIAELPAVINFDLPFNAEDYVHRIGRTGRAGASGDALSL
CSPNERKQLADIEKLIKRPLDVQRLTVEAPVRHHHEERAPRRERSSEGREDRGSRRRTGA
SSGSYDRPHHHRAQPVDDFFLKPYEPSPSSVRKIEEAAAAAAPQKPASKQPLAALLGGFG
MPRKSSSST