Protein Info for BPHYT_RS06150 in Burkholderia phytofirmans PsJN

Annotation: aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 PF00171: Aldedh" amino acids 19 to 470 (452 residues), 452.4 bits, see alignment E=7.9e-140

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1249)

MetaCyc: 41% identical to 3-sulfolactaldehyde dehydrogenase (Alkalicoccus urumqiensis)
RXN-17101 [EC: 1.2.1.97]

Predicted SEED Role

"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.2.1.97 or 1.5.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T253 at UniProt or InterPro

Protein Sequence (478 amino acids)

>BPHYT_RS06150 aldehyde dehydrogenase (Burkholderia phytofirmans PsJN)
MLQKTYPYYLANVAVAANTDLEVTDKFSGEVATRVAMADAAAIDKAIGHAVDAMPALRAF
PPFKRQAVLEHCVKRFRERYDELALALCIEAGKPINDSKGEVTRLIDTFKVAAEESVRID
GEIVNLEISPRAKGYHGYVKRVPIGPCSFISPFNFPLNLTAHKVAPAIAAGVPFVLKPAS
RTPVGALIMGEILAETDLPKGAFSILPAHRDGADLFTTDERFKLLSFTGSPAVGWDLKKK
AGKKKVILELGGNAAAIVDGDQGEKLDYVVERLAFGAFYQSGQSCIGVQRILVHTKIYDA
LREKLIAKTKSLVMGDPKDEKTFVGPMISESESKRLAGWMDSAVQAGAKIVAGGKVDGAM
FEATLLEGVKRDTDLYRKEAFGPVAILERFDDFGAALATVNDSDFGLQAGVFTDSLAHAH
RAWDELDVGGVVINDVPSFRVDNMPYGGVKDSGLGREGVRYAIEDMTELRLMVMRETW