Protein Info for BPHYT_RS06095 in Burkholderia phytofirmans PsJN

Annotation: esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01849: polyhydroxyalkanoate depolymerase, intracellular
  • amino acids 2 to 411 (410 residues), 601.6 bits, E=3.3e-185
• PF06850: PHB_depo_C
  • amino acids 211 to 411 (201 residues), 319 bits, E=5.6e-100

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 100% identity to bpy:Bphyt_1238)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Alkaloid biosynthesis II
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Glycerophospholipid metabolism
• Retinol metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Sequence Analysis Tools

See B2T242 at UniProt or InterPro

Protein Sequence (425 amino acids)

>BPHYT_RS06095 esterase (Burkholderia phytofirmans PsJN)
MLYQLHEFQRAMLSPLTAWAQAASKSFANPASPLAYVPGATRLSAGYELLYRLGKDYEKP
EFNLHQIVKDGHNIPIIEQTIIEKPFCRLMRFKRFADDSDAVTQLKDEPVVLVCAPLSGH
HATLLRDTVKTLLQDHKVYLTDWIDARMVPLEAGQFRLDDYVAYIQEFIRHIGAKNLHVI
SVCQPTVPVLAAISLLASRGEDTPRTMTMMGGPIDARKSPTSVNSLATQHSYEWFENNVI
FTVPPNYPGVGRKVYPGFLQHTGFVAMNPERHAASHWDYYQSLLRGDEDDAEAHRRFYDE
YNAVLDMDADYYLDTIRVVFQEFSLAEGTWDVSGERVKPQDITKTALFTIEGELDDISGD
GQTYAAHDLCTGIPEEHKRHFTAEKCGHYGIFSGRRWRTIIYPQLRDFILEHNKAPKVAK
EKVEA