Protein Info for BPHYT_RS05935 in Burkholderia phytofirmans PsJN

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 774 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 21 to 713 (693 residues), 782.4 bits, see alignment E=2e-239 PF00521: DNA_topoisoIV" amino acids 42 to 500 (459 residues), 480.5 bits, see alignment E=2.6e-148

Best Hits

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 100% identity to bpy:Bphyt_1206)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T210 at UniProt or InterPro

Protein Sequence (774 amino acids)

>BPHYT_RS05935 DNA topoisomerase IV subunit A (Burkholderia phytofirmans PsJN)
MDDDNTLDLFTEPPPPEGDFLTLGNYAERAYLDYAVSVVKGRALPDVCDGQKPVQRRILF
AMNDMGLSDNAKPVKSARVVGDVLGKYHPHGDQSAYDALVRLAQDFSMRYPLIDGQGNFG
SRDGDGAAAMRYTEARLTPIAKLLLDEINQGTVDFMPNYDGSSEEPRLLPARLPFVLLNG
ASGIAVGLATEIPSHNLREVAAATVAMIRNPKIEHAELMQHIAGPDFPGGAQIISSDAEI
SAAYESGRGSLKVRARWKIEELARGQWQLVITELPPNTAAQKVLEEIEEQTNPKIKLGKK
ALTPEQLQTKQTMLALLDAVRDESGRDAPVRLVFEPKSSRIDQTDFVNTLLAYTSLESNA
SLNMVMVGGDGRPRQKGLSEILHEWIAFRFATVTRRTRHRLSKVDDRIHILEGRMIVFLN
IDEVIRIIRESDEPKVALMAAFGLSDRQAEDILEIRLRQLARLEKIKIEKELAELRDEKA
KLEELLGSESAMKRLLIKEIEGDAKQYGDERRTLIQQEKRATFEARVVDEPVTVVVSQKG
WVRALKGHGLDVAGFTFKAGDGLYAAFQCRTPDTLVAWGSNGRVYSVAVAMLPGGRGDGV
PVTSLIELESGSHLLHYYAASAEQALLLASSNGFGFVAKVGDMVSRVKAGKSFMTIDAGA
APLKPMPMLPDATHIACLSSGGRLLVFGIDEMKTLSGGGRGVTLMALDDKETLVQALSIN
NAGVVLIGTRRGGRVDEEKVSGAALAPHIGKRARKGRAPESKMKLEGMRPVLAA