Protein Info for BPHYT_RS05930 in Burkholderia phytofirmans PsJN

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 PF02518: HATPase_c" amino acids 38 to 177 (140 residues), 49.1 bits, see alignment E=1.5e-16 PF00204: DNA_gyraseB" amino acids 257 to 403 (147 residues), 113.8 bits, see alignment E=1.3e-36 PF01751: Toprim" amino acids 431 to 544 (114 residues), 47.5 bits, see alignment E=3.2e-16 PF00986: DNA_gyraseB_C" amino acids 587 to 651 (65 residues), 76.7 bits, see alignment E=2.4e-25

Best Hits

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 100% identity to bpy:Bphyt_1205)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T209 at UniProt or InterPro

Protein Sequence (661 amino acids)

>BPHYT_RS05930 DNA topoisomerase IV subunit B (Burkholderia phytofirmans PsJN)
MSTKKPNAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGRQI
TVTLHADHSVSVEDDGRGIPFGLHPEEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLH
GVGVSVTNALSTRLDVTVWRDGKIAELSFANGDVAKELEVRPAAKGDKKSGTRVTAWANP
KYFDSPNLPLGELQRLLRSKAVLLPGVEVTLINEKTGEQQTWKYEDGLRGYLLEGMNGSD
LLIPLFEGERYAENSRASEETFAEGEGAAWVVAWSEEGTLMRESYVNLIPTPAGGTHESG
LRDGLYQAVKSFVELHNLQPKGVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDA
VKLVSSFARPALELWLNQHVELGKKLADLVIKQAQARTRAGQKVEKRKSSGVAVLPGKLT
DCESTEIGRNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVH
DISVAIGVDPHSPDDKVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGH
VCVARPPLFRVDAPARGKKPAQKLYALDEGELEAILDKLRKDGVRDTQWSISRFKGLGEM
SAEQLWDTTMNPDTRRLSPVALGALDYDATVARMTMLMGKGEAASRRSWLEEKGNEVEAD
I