Protein Info for BPHYT_RS05425 in Burkholderia phytofirmans PsJN

Annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 PF00970: FAD_binding_6" amino acids 347 to 444 (98 residues), 28.5 bits, see alignment E=3.2e-10 PF00175: NAD_binding_1" amino acids 455 to 566 (112 residues), 45.5 bits, see alignment E=2.1e-15 PF00111: Fer2" amino acids 624 to 680 (57 residues), 40.5 bits, see alignment 4.4e-14

Best Hits

KEGG orthology group: K07006, (no description) (inferred from 100% identity to bpy:Bphyt_1107)

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.17

Use Curated BLAST to search for 1.14.12.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1G6 at UniProt or InterPro

Protein Sequence (700 amino acids)

>BPHYT_RS05425 oxidoreductase (Burkholderia phytofirmans PsJN)
MSSSTVPSSPGWDLERSPFHEGELAVQERVGVREKIDAQGRRAVRRYLTAQQREFFLMLP
YVFMASVDSSGRPWASFLVGEPGFVNPTGDVTLTVRARALYGDPLNDTLVPGAEIALLGV
QFHTRRRNRVIGTVGALLPDGFTIDVRQTLGICPQYIQGRELSFNRDPLLPEPRPVHRNS
RLDASASAIISKADTYFVASVLLQEGDPVATGADVSHRGGRPGFVRIDDDHTLTAPDFVG
NFIFNTIGNWQFDNHAGLMFIDFDSGAMLYIAAHAEVLWDGPELHAFNGAQRLFRYHIDE
VCRVEASLPCTFTAPDYSPLLERTGHWDIVAKTLEAERLRTVWRPFKVLETIDESATIRS
FILEPADGSGLVGYQAGQYLPIRLQPPGWAEPTIRTYTLSDASNGRTYRISVKREGKGGC
SDFLHDHIVVGDTIETLAPRGAFVFDEAAGRPLALISAGVGITPLIAMLNSQLVNDGRTL
LHKPIYCIHSATNSKHRAFAAHLAKKGELHANLTLHVAFSRPSEDDVLGKTHQSEGYIDR
ALLRRVLPLDDYEVYLCGPPGFMQSVYDALLSLGVRDSRIHLESFGPASVSRRIERTVEV
DNSEGVVVTFAKSGRNAIWRPKVGSLLELAEANGLKPLYACRSGSCGTCVTRVVKGEVDY
TEPPAHDVEPGEALICIARPHPGPHLEDGTQNREGVTLDI