Protein Info for BPHYT_RS04955 in Burkholderia phytofirmans PsJN

Annotation: methionyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 TIGR00398: methionine--tRNA ligase" amino acids 19 to 531 (513 residues), 528.1 bits, see alignment E=2.6e-162 PF09334: tRNA-synt_1g" amino acids 19 to 412 (394 residues), 481.1 bits, see alignment E=8e-148 PF00133: tRNA-synt_1" amino acids 22 to 77 (56 residues), 37.2 bits, see alignment 5.4e-13 PF08264: Anticodon_1" amino acids 433 to 526 (94 residues), 28.7 bits, see alignment E=4e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 579 to 711 (133 residues), 137.8 bits, see alignment E=2.1e-44 PF01588: tRNA_bind" amino acids 611 to 708 (98 residues), 88.5 bits, see alignment E=8.9e-29

Best Hits

Swiss-Prot: 97% identical to SYM_PARXL: Methionine--tRNA ligase (metG) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to bpy:Bphyt_1015)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.10

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T177 at UniProt or InterPro

Protein Sequence (711 amino acids)

>BPHYT_RS04955 methionyl-tRNA synthetase (Burkholderia phytofirmans PsJN)
MSAPVTDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYV
GADDTHGTPIMLRAEKEGLTPKQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRILSEN
VYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCGSTYLPT
ELINPYSVVSGATPIRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGS
TAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQS
VIDTGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKRF
DGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQA
KDPANGVALHETCTVSIEAFRLLSLALKPVLPKLAEAVEGFLGVEPLVWADANVPLSSTR
PINAYKHLMTRVDSKQIDALIAANRDSLQATPEAAAPADAKGKSKAAKTAAAKEDETPGI
ISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKL
TVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK