Protein Info for BPHYT_RS04795 in Burkholderia phytofirmans PsJN
Annotation: cytosol aminopeptidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AMPA_PARPJ: Probable cytosol aminopeptidase (pepA) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to bpy:Bphyt_0984)MetaCyc: 51% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- γ-glutamyl cycle (5/6 steps found)
- glutathione degradation (DUG pathway) (2/2 steps found)
- muropeptide degradation (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T146 at UniProt or InterPro
Protein Sequence (508 amino acids)
>BPHYT_RS04795 cytosol aminopeptidase (Burkholderia phytofirmans PsJN) MDFSIKACDWTKGSSNGFLTGKSDCIVIGVFESQTLSGAALEIDEATRGLLTRIIKAGDM DGKAGTTLFLHEVSGIGASRVLLVGLGKQDAFNQKAYGDAVRAAWRALLGTKIVQVTFTL AQAPILERTADWGVRAAILALRELTYKFTQMKSKPENAARALKRIVFSVNTGDEKAAKLA AKQGAALANGMDLTRDLGNLPSNVCTPTYLANTAKKLAKDWKLKVEVLGEKQCEALKMGS FLSVTAGSVEPAQFIVLQYQGGAAKAAPVVLVGKGVTFDTGGISLKPGEGMDEMKYDMCG AGSVLGTLRAVAEMGLKLNVVGIIPAVENMPSATATKPGDIVTSMKGLTIEVLNTDAEGR LILCDALTYAERFKPAAVIDIATLTGACIIALGHHNSGLFSKDDALAGELLDASREASDP AWRLPLDDEYQDQLKSNFADLANIGGRPAGSVTAACFLSRFTDAYPWAHLDIAGTAWKSG AAKGATGRPVPLLAQFLIDRAADGRATQ