Protein Info for BPHYT_RS04775 in Burkholderia phytofirmans PsJN

Updated annotation (from data): 2-deoxy-D-ribonate 3-dehydrogenase
Rationale: Important for deoxyribonate utilization. Similar to the deoxyribonate dehydrogenase of P. simiae (PS417_07245)
Original annotation: 3-ketoacyl-ACP reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 263 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details PF08659: KR" amino acids 15 to 163 (149 residues), 25.3 bits, see alignment E=2.6e-09 PF00106: adh_short" amino acids 15 to 197 (183 residues), 155.3 bits, see alignment E=2.9e-49 PF13561: adh_short_C2" amino acids 19 to 258 (240 residues), 184.6 bits, see alignment E=4.6e-58

Best Hits

Swiss-Prot: 36% identical to GDH_AGRFC: Galactitol 2-dehydrogenase (Atu3164) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0980)

MetaCyc: 60% identical to 2-deoxy-D-ribonate dehydrogenase (Pseudomonas simiae)
1.1.1.M55 [EC: 1.1.1.M55]

Predicted SEED Role

"SHORT CHAIN DEHYDROGENASE"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.M55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T142 at UniProt or InterPro

Protein Sequence (263 amino acids)

>BPHYT_RS04775 2-deoxy-D-ribonate 3-dehydrogenase (Burkholderia phytofirmans PsJN)
MKTIDLLRPTPGLRVLISGAAAGIGAAIAQAFLDVGANVYICDVDPAAIDRARTAHPQLH
AGVADVSDCAQVDRIIDDARSKLGGLDLLINNAGIAGPTGAVEDLDPAEWERTIGTNLNS
QFYFLRKAVPLLKETSANPGIIAMASVAGRLGYAFRTPYAASKWAIVGMVKSLAIELGPN
NVRVNAILPGVVEGERMDRVISARAESLGIGFDQMKGEYLQKISLRRMVTVHDVAAMALF
LASPAGQNISGQAISVDGNVEYL