Protein Info for BPHYT_RS04745 in Burkholderia phytofirmans PsJN

Annotation: sulfate adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 13 to 218 (206 residues), 142.8 bits, E=1e-45
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 13 to 433 (421 residues), 524 bits, E=1.4e-161

Best Hits

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 100% identity to bxe:Bxe_A3660)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (4/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

See B2T136 at UniProt or InterPro

Protein Sequence (438 amino acids)

>BPHYT_RS04745 sulfate adenylyltransferase (Burkholderia phytofirmans PsJN)
MSGIHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEI
DLSLLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAII
LVDATRVTFVDGVAQLLPQTKRHSAIVKLLGLQHAIVAINKMDLVDYSEQRFNEIRDAYV
ELARQLGLNDVRFVPVSALKGDNIVTASESMPWYAGEPLLDLLEALPVEVPTGQALRFPV
QWVARQDGSQADDFRGYMGRVEAGEVKLGDTIVVLPANREATVAEIIAPVPGGTAAVDRA
FAGQTVTIRLAEDVDVSRGDTFVLREAAPEPAKKLEADLCWFDDTPLSTQRKYLLKQTTN
TVFARIGAIKEVLDVHTLSQATDRKELAMNDIGRVALTLQKPLVCDVYDSHQGTGAFVLI
DEATHHTVAAGMIRAFSA