Protein Info for BPHYT_RS04735 in Burkholderia phytofirmans PsJN

Annotation: phosphoadenosine phosphosulfate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01507: PAPS_reduct
  • amino acids 34 to 206 (173 residues), 145.7 bits, E=7.7e-47
• TIGR02055: adenylylsulfate reductase, thioredoxin dependent
  • amino acids 37 to 230 (194 residues), 288 bits, E=1.8e-90

Best Hits

Swiss-Prot: 84% identical to CYSH_BURCE: Thioredoxin-dependent 5'-adenylylsulfate reductase (cysH) from Burkholderia cepacia

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 100% identity to bpy:Bphyt_0972)

MetaCyc: 54% identical to assimilatory adenylyl-sulfate reductase (Allochromatium vinosum)
Adenylyl-sulfate reductase (glutathione). [EC: 1.8.4.9]

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10)" in subsystem Cysteine Biosynthesis (EC 1.8.4.10, EC 1.8.4.8)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• selenate reduction (2/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.4.10 or 1.8.4.8 or 1.8.4.9

Sequence Analysis Tools

See B2T134 at UniProt or InterPro

Protein Sequence (248 amino acids)

>BPHYT_RS04735 phosphoadenosine phosphosulfate reductase (Burkholderia phytofirmans PsJN)
MSEDQALTPELAAKVERLDALLDSIAARHENVKLASSLAAEDMLLTHAILSRGVKIGIFS
LNTGRLHAETLGMLDRVKERYGYDIEQFHPQAAAVEEYVGSHGLNAFYESVDLRKRCCEI
RKVEPLNRALSDVSAWVTGQRREQSVTRAELHAEEHDAARNIAKFNPLTDWTEAEVWAYL
KAFDVPVNPLHARGYPSIGCEPCTRAVRPGEDSRAGRWWWESRDTKECGLHITTITPIAV
DRSANASA