Protein Info for BPHYT_RS04545 in Burkholderia phytofirmans PsJN

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 transmembrane" amino acids 226 to 247 (22 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 42 to 252 (211 residues), 201.2 bits, see alignment E=1.7e-63 PF09850: DotU" amino acids 43 to 248 (206 residues), 203.5 bits, see alignment E=2.9e-64 TIGR03350: type VI secretion system peptidoglycan-associated domain" amino acids 277 to 412 (136 residues), 160.2 bits, see alignment E=2.8e-51 PF00691: OmpA" amino acids 308 to 406 (99 residues), 55.2 bits, see alignment E=7.5e-19

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to bpy:Bphyt_0935)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0Q2 at UniProt or InterPro

Protein Sequence (414 amino acids)

>BPHYT_RS04545 membrane protein (Burkholderia phytofirmans PsJN)
MEKKTGDSASGAKPELPATLLASIPAGEPQAARLQAVEDATNPLLEAARPLLRALADMPD
NLDLTAIDQLHLLLKQEVRTFQRLCEQANIRRDHMIGARYCLATALDEAAGKTVWGQRET
GVEWLRKGLATEFHEDRQGGDKVYLLIGRLMNEPREHLDLLEVIYRILSLGFMGRYRHEA
DGARKHDAVRQRLYNEIQTQRGVVPIALSPRVQSDSRGQRLSVYDFPVRITFAVLGLILL
GLFGWFKYHLLNHGASVEKQITDIGHLTPPAPRLPHLKELLKNEIAAGTVSVDEDARHSS
VTFRGDAMFPPGGVNVNASMGPLIAKIASEIGKVPGKVTVTGHTDNLPIRSRQFASNDAL
SEERATQVMQMLQASGVAAARLEAIGGGEANPIGDNATAQGRAQNRRVEITVTP