Protein Info for BPHYT_RS04510 in Burkholderia phytofirmans PsJN

Annotation: urease subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 568 PF00449: Urease_alpha" amino acids 4 to 120 (117 residues), 189.1 bits, see alignment E=4.6e-60 TIGR01792: urease, alpha subunit" amino acids 5 to 568 (564 residues), 991.7 bits, see alignment E=5.9e-303 PF01979: Amidohydro_1" amino acids 126 to 454 (329 residues), 283.4 bits, see alignment E=4.3e-88

Best Hits

Swiss-Prot: 93% identical to URE1_BURL3: Urease subunit alpha (ureC) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 100% identity to bpy:Bphyt_0928)

MetaCyc: 75% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0P5 at UniProt or InterPro

Protein Sequence (568 amino acids)

>BPHYT_RS04510 urease subunit alpha (Burkholderia phytofirmans PsJN)
MTLRIGRRAYAEMFGPTSGDRVRLADTELLIEVERDFTTYGEEVKFGGGKVIRDGMGQSQ
RVHAEVVDTVVTNAVILDHWGIVKADIGIKDGRIAAIGKAGNPDIQPNVTIAIGASTEVI
AGEGLIVTAGGIDTHIHFISPQQIEEALASGVTTMLGGGTGPATGTNATTCTPGPWHLER
MLQAADGYPMNLGFLGKGNVSLPQPALEQIAAGAIGLKLHEDWGTTPAAIDNCLSVADDT
DTQVAIHTDTLNEAGFVEATVAAFKGRTIHTYHTEGAGGGHAPDIIKVCGEANVLPSSTN
PTRPYTVNTLEEHLDMLMVCHHLDPSIAEDIAFAESRIRRETIAAEDILHDLGALSMLSS
DSQAMGRVGEVIIRTWQTAHKMKVQRGALPEDNARHDNFRAKRYVAKYTINPAITHGIAH
EVGSIEPGKWADLVFWEPAFFGIKPSLILKGGMIAMAQMGDPNASIPTPQPVHYREMFAT
RGGALGRTSLTFVSQIAADAGVGERYGLNKRIVAVRNCRNISKADMIHNAWRPAISVDPE
TYQVIADGQLLTCEPATVLPMAQRYFLF