Protein Info for BPHYT_RS04485 in Burkholderia phytofirmans PsJN

Annotation: 3-deoxy-D-manno-octulosonic acid transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04413: Glycos_transf_N
  • amino acids 34 to 212 (179 residues), 222.1 bits, E=4e-70
• PF00534: Glycos_transf_1
  • amino acids 312 to 412 (101 residues), 29.9 bits, E=3.9e-11

Best Hits

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 100% identity to bpy:Bphyt_0923)

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
• Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
• Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
• Kdo transfer to lipid IV_A (generic) (2/2 steps found)
• Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
• Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
• Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (15/25 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Sequence Analysis Tools

See B2T0P0 at UniProt or InterPro

Protein Sequence (438 amino acids)

>BPHYT_RS04485 3-deoxy-D-manno-octulosonic acid transferase (Burkholderia phytofirmans PsJN)
MLRAIYHALWWIIAPLAVLRLLIRSRKERGYREHIGERFGYSRGRLPEDDAPLIWVHAVS
VGETRAAQPLIDALMKARPDARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRF
LRAWRPSLGLVMETEVWPTLIDECRGADVPLVLTNARMSARSYKRAAKFGSATKDVFGGF
ARVLAQSPSDAERLTALGARNVAVLGNLKFDMSTPPELVARGHAWRAAIGARPVWVAAST
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLARRSVWAPDAKVAS
AAAAGGGVTALPSDVNVLLGDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVPV
LIGPHVFNFTQATADAVAAGAAVQVKDPADLGRALRELFGDKARRLAMGGAASAFAARHR
GATARTVDVLMALLPEEG