Protein Info for BPHYT_RS04455 in Burkholderia phytofirmans PsJN

Annotation: glycosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13477: Glyco_trans_4_2" amino acids 13 to 153 (141 residues), 57 bits, see alignment E=8e-19 PF13579: Glyco_trans_4_4" amino acids 25 to 178 (154 residues), 44.4 bits, see alignment E=6.8e-15 PF13439: Glyco_transf_4" amino acids 30 to 152 (123 residues), 28.9 bits, see alignment E=3.2e-10 PF00534: Glycos_transf_1" amino acids 197 to 355 (159 residues), 101.4 bits, see alignment E=1.3e-32 PF13692: Glyco_trans_1_4" amino acids 200 to 340 (141 residues), 98.4 bits, see alignment E=1.4e-31 PF13524: Glyco_trans_1_2" amino acids 282 to 369 (88 residues), 39 bits, see alignment E=2.3e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0917)

Predicted SEED Role

"Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase" in subsystem N-linked Glycosylation in Bacteria (EC 2.4.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0N4 at UniProt or InterPro

Protein Sequence (382 amino acids)

>BPHYT_RS04455 glycosyl transferase (Burkholderia phytofirmans PsJN)
MKPALKSTPALRITLVCNTAWAIYTYRQGLIRMLVGRGVDVTIVAPRDRTFELLTAMGCR
CIDLPVASKSTSPRDDLRTLYALYRHYRSIRPHVVFHYTIKPNIYGSIAAKLAGVQSVAV
TTGLGYVFIQHSRAAQVAKKLYRFAFRFPREIWFLNRDDQAAFVEQNLLVHPERARLLHG
EGVDLVQFAFMPLPERTDYRFVLIGRLLWDKGVGEYVEAARRLRERYPHARFQLLGPVGV
DNPSAITREEVAAWEQEGIIEYLGEAHDVRPLIAEADCVVLPSYREGVPRTLMEASAIGR
PIVATDVPGCREVVADGVNGLLCEVRNAESLAAALARMLDMSGAERRAMAERGRKKVAEE
FDERVVVETYKDLVQKMTGVLL