Protein Info for BPHYT_RS04225 in Burkholderia phytofirmans PsJN

Annotation: Holliday junction resolvase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 146 PF03652: RuvX" amino acids 10 to 137 (128 residues), 136.7 bits, see alignment E=3.3e-44 TIGR00250: putative transcription antitermination factor YqgF" amino acids 12 to 137 (126 residues), 99.7 bits, see alignment E=6.8e-33

Best Hits

Swiss-Prot: 100% identical to YQGF_PARPJ: Putative pre-16S rRNA nuclease (Bphyt_0869) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K07447, putative holliday junction resolvase [EC: 3.1.-.-] (inferred from 100% identity to bpy:Bphyt_0869)

Predicted SEED Role

"Putative Holliday junction resolvase YqgF"

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0I5 at UniProt or InterPro

Protein Sequence (146 amino acids)

>BPHYT_RS04225 Holliday junction resolvase (Burkholderia phytofirmans PsJN)
MSLPAGREATLLAFDYGEKRIGVAVGNSLTRRARPLVIVQNRSREYRFEAVGKLIAEWKP
DALVVGLPFHPDGAPHEMTQLAKRFGNQLNGRFNLPVTWVDERYSSVEAKAEIRAGNGRA
DMLDAEAASIILQQYLDGLSDDHEFH