Protein Info for BPHYT_RS04200 in Burkholderia phytofirmans PsJN

Annotation: molecular chaperone GroEL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 TIGR02348: chaperonin GroL" amino acids 3 to 527 (525 residues), 842 bits, see alignment E=8.1e-258 PF00118: Cpn60_TCP1" amino acids 23 to 521 (499 residues), 281.1 bits, see alignment E=8.5e-88

Best Hits

Swiss-Prot: 100% identical to CH60_PARPJ: 60 kDa chaperonin (groL) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K04077, chaperonin GroEL (inferred from 100% identity to bpy:Bphyt_0864)

MetaCyc: 75% identical to chaperonin GroEL (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Heat shock protein 60 family chaperone GroEL" in subsystem GroEL GroES or Staphylococcal pathogenicity islands SaPI

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0I0 at UniProt or InterPro

Protein Sequence (546 amino acids)

>BPHYT_RS04200 molecular chaperone GroEL (Burkholderia phytofirmans PsJN)
MAAKDVVFGDSARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEI
ELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQSIVREGMKYVASGMNPMDLKRGI
DKAVTAAIEELRKISKPCTTNKEIAQVGAISANSDSSIGDRIAEAMDKVGKEGVITVEDG
KSLQDELDVVEGMQFDRGYLSPYFINNPDKQVAVLDNPFVLLHDKKVSNIRDLLPVLEQV
AKAGRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLEDIAILTGGQV
IAEETGLTLEKATLAELGQAKRIEVGKENTTIIDGAGEAANIEARVKQVRTQIEEATSDY
DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVAGGGVALI
RARTAIAGLKGANADQDAGIKIVLRAMEEPLRQIVTNGGEEASVVVAAVAAGQGNYGYNA
ATGEYVDLVDAGVVDPTKVTRTALQNAASVAGLLLTTDAAVCELPKEDAPMAGGMPGGMG
GMGMDM