Protein Info for BPHYT_RS03720 in Burkholderia phytofirmans PsJN

Annotation: malonic semialdehyde reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 186 (185 residues), 168 bits, E=3.8e-53
• PF08659: KR
  • amino acids 3 to 163 (161 residues), 46 bits, E=1.1e-15
• PF01370: Epimerase
  • amino acids 3 to 175 (173 residues), 28.2 bits, E=2.5e-10
• PF13561: adh_short_C2
  • amino acids 7 to 221 (215 residues), 117.3 bits, E=1.8e-37

Best Hits

Swiss-Prot: 70% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 100% identity to bpy:Bphyt_0764)

MetaCyc: 68% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (16/18 steps found)
• L-threonine degradation III (to methylglyoxal) (3/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• aminopropanol phosphate biosynthesis II (2/4 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

See B2T082 at UniProt or InterPro

Protein Sequence (248 amino acids)

>BPHYT_RS03720 malonic semialdehyde reductase (Burkholderia phytofirmans PsJN)
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQELADELGDALLPYELDVRDRAA
VEAVPASLPADFAAIDVLVNNAGLALGIEPAQKASLDEWNTMIETNCTGLVQVTHALLPG
MVERNRGHIFNLGSAAGSWPYAGGNVYGATKAFVHQFSLNLRADVAGTALRVTNVEPGLC
GGTEFSNVRFRGDDVKAGKMYENVQPLTPDDIADSIYWIATRPAHVNINTIELMPVAQSF
SALSVHRG