Protein Info for BPHYT_RS03555 in Burkholderia phytofirmans PsJN

Annotation: glutamine-synthetase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 PF03710: GlnE" amino acids 48 to 255 (208 residues), 208.1 bits, see alignment E=2.2e-65 amino acids 552 to 791 (240 residues), 218.2 bits, see alignment E=1.9e-68 PF08335: GlnD_UR_UTase" amino acids 283 to 426 (144 residues), 109.7 bits, see alignment E=1.8e-35 amino acids 813 to 901 (89 residues), 30.8 bits, see alignment E=4.2e-11

Best Hits

Swiss-Prot: 78% identical to GLNE_BURMA: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Burkholderia mallei (strain ATCC 23344)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to bpy:Bphyt_0729)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXB4 at UniProt or InterPro

Protein Sequence (930 amino acids)

>BPHYT_RS03555 glutamine-synthetase adenylyltransferase (Burkholderia phytofirmans PsJN)
MTDATLLSSNYSHYAARAAAARPQLVARVAALASGPLTRERIDARFDALCAEAAGATDAP
LNEDALKRALRQLRTEVFCAVMERDLAGEADVAEVTGAMTDLAETTIQRALAVLSAELET
LYGEPRGPQGERLSLGVVGMGKLGGRELNVSSDIDLIFIYEEDGETSGGQRSPIATQEFF
TRLGKRLIGALAEVTGDGYVFRVDMRLRPNGDSGPLVCSLGMLEEYFYVQGREWERYAWI
KGRLVSEGASDAAQRLQKQLDAIVTPFVYRRYLDFGVISAIRALHLQIRQEAQRRASMRP
DKADDIKLGRGGIREIEFSAQVFQLIRGGQDAGFRVRPTLAVLRHAATHGLLDTSVCVKL
SQAYRFLRELEHRLQYRNDAQTHAMPVDPEDRSALAHAMGCDDYAALMTKLDAHREFVEQ
QFDQIFADKVNGRDGCGAPEDGAAAWVWSSALADDSADEALQSRLVELGVAEPGELVARL
RAIWQSSRYAGLAERSRQRFDIVAQRALEAARTLEPAERRGDTVARFFDLLEAVSRRGAY
LALLTEYPQALHRVLSVLGGSRWAAGYLIRHPQLLDELLDDEAINSPFDWPEFKRTLRLR
LAAADGVEQQMDLLRHAHQAEVFRILLIDLAGKLSVEHVSDRLSELADAVLDVTLQAVWN
QLPKRHRDVPRFAVIAYGKLGGKELGYASDLDLIFLYDDPDDAAADVYATYTRRLITWLT
TATGAGTLFDVDLRLRPNGESGLLVTDLDAFRRYQLREGDAANTAWVWEHQALSRARYCA
GDAEIGAKFEAIREQVLTTPREAAPLMKEIVEMRARVEAGHPNHTALFDLKHDSGGMVDI
EFTVQYWVLLHAASDPELIRNTGNIALLREVSRFGLMSEAEAETVGAAYRTYRKLQHQLR
LDGMEKARVEPALVAKEREAVLALWKRVFG