Protein Info for BPHYT_RS03540 in Burkholderia phytofirmans PsJN

Annotation: peptidase C69

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 PF01523: PmbA_TldD_1st" amino acids 44 to 104 (61 residues), 58.3 bits, see alignment E=1.1e-19 PF19290: PmbA_TldD_2nd" amino acids 137 to 245 (109 residues), 73.7 bits, see alignment E=2.7e-24 PF19289: PmbA_TldD_3rd" amino acids 253 to 486 (234 residues), 233 bits, see alignment E=3.5e-73

Best Hits

Swiss-Prot: 58% identical to TLDD_SHIFL: Metalloprotease TldD homolog (tldD) from Shigella flexneri

KEGG orthology group: K03568, TldD protein (inferred from 100% identity to bpy:Bphyt_0726)

MetaCyc: 58% identical to metalloprotease subunit TldD (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldD protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXB1 at UniProt or InterPro

Protein Sequence (488 amino acids)

>BPHYT_RS03540 peptidase C69 (Burkholderia phytofirmans PsJN)
MNIIEPGIRNLATAKDILLTPYGLDEALLTRTLAEIFTHKIDYADLYFQYTRSEAWSLEE
GIVKSGSFSIDQGVGVRAVSGDRTAFAYSDDLSPEAIRQAAIATRAIGKAGGGKQKIKVA
SSLTGIAGRDLYLPADPLHSLDATAKVKLLERIEKMARGRDPRIQQVMAGLAGEYDVVLV
ARSDGGFAADIRPLVRVSVTVIAEQNGRREIGSGGGGGRFDYGYFTDEILSSYVDDAVHA
ALVNLDARPAPAGAMTVVLGPGWPGVLLHEAIGHGLEGDFNRKGSSAFAGRIGEQVAAKG
VTVVDDGTLPNRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTLNARLMKMPVTGNARRE
SYAALPMPRMTNTYMLNGDKDPQEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMI
ENGKVTYPVKGATLIGSGPESLKYVTMIGNDMKLDSGVGVCGKEGQSVPVGVGQPTLRIE
KMTVGGTA