Protein Info for BPHYT_RS03515 in Burkholderia phytofirmans PsJN

Annotation: D-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 PF01565: FAD_binding_4" amino acids 52 to 189 (138 residues), 145.1 bits, see alignment E=1.2e-46 PF02913: FAD-oxidase_C" amino acids 226 to 466 (241 residues), 224.4 bits, see alignment E=1.9e-70

Best Hits

Swiss-Prot: 53% identical to LDHD_MOUSE: Probable D-lactate dehydrogenase, mitochondrial (Ldhd) from Mus musculus

KEGG orthology group: K00102, D-lactate dehydrogenase (cytochrome) [EC: 1.1.2.4] (inferred from 100% identity to bpy:Bphyt_0721)

MetaCyc: 52% identical to D-lactate dehydrogenase (cytochrome c) monomer (Arabidopsis thaliana col)
D-lactate dehydrogenase (cytochrome). [EC: 1.1.2.4]; 1.1.2.4 [EC: 1.1.2.4]; 1.1.2.4 [EC: 1.1.2.4]

Predicted SEED Role

"D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXA6 at UniProt or InterPro

Protein Sequence (471 amino acids)

>BPHYT_RS03515 D-lactate dehydrogenase (Burkholderia phytofirmans PsJN)
MNHPVPPAPLRRPFPAELLNALKAAFADRVSVSEVVRTHHGRDESPFDPQLPDAVVFART
TEDVQTIVKLCGQYNVPIIPYGNGSSLEGHLLAVQGGVSIDLSEMNRVLSINAEDLTVTV
EPGISRKQLNEALRDTGLFFPIDPGADASIGGMSATRASGTNAVRYGTMRENVLGLTVVL
ADGRVIKTGTRARKSSAGYDLTRMFVGSEGTLGVITEITVRLYPQPEAVSAAVCAFPSMG
AAVRAVIETIQMGVPIARVEFVDSLAIRSINRHSNLTLREAPTLFFEFHGTEAGVKEQAE
LVQEIAAQNAGEGFEWATRPEDRSRLWNARHNAYFAMLQLKPGCRAVTTDVCVPISRLAE
CVEETEQDLQASPLPCPIVGHVGDGNFHVAILIDPNKPEELVEAERLNHRIVQRALRMDG
TCTGEHGVGLHKMGFLLEEHGEVAVDTMRSIKHALDPHNLMNPGKIFTWAA