Protein Info for BPHYT_RS03510 in Burkholderia phytofirmans PsJN
Annotation: FAD-binding protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to GLCD_ECOL6: Glycolate oxidase subunit GlcD (glcD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 100% identity to bpy:Bphyt_0720)MetaCyc: 60% identical to glycolate dehydrogenase, putative FAD-linked subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]
Predicted SEED Role
"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)
MetaCyc Pathways
- glycolate and glyoxylate degradation II (2/2 steps found)
- glycolate and glyoxylate degradation I (3/4 steps found)
- superpathway of glycol metabolism and degradation (5/7 steps found)
- photorespiration I (6/9 steps found)
- photorespiration III (6/9 steps found)
- glycolate and glyoxylate degradation III (1/3 steps found)
- photorespiration II (6/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.3.15, 1.1.99.14
Use Curated BLAST to search for 1.1.3.15 or 1.1.99.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2SXA5 at UniProt or InterPro
Protein Sequence (497 amino acids)
>BPHYT_RS03510 FAD-binding protein (Burkholderia phytofirmans PsJN) MNAPAELTAEVLAQRQREVVQALMAVLPTHCLLFREEDTVAYECDGLAAYRRLPLAVALP ETESQVQRIVQICHRLDVPIVPRGAGTGLSGGAMPIRHGVVVSLARFRKIVEVDSYARTA TVQPGVRNLSISEAAAPYGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGLTVHNVLRV RAVTMEGEIVEFGSLAPDAPGLDLLAVLIGSEGMFAIVTEVTVKLIPKPQTAQVIMASFD DVVKGGDAVAGIIAAGIIPAGLEMMDKPATRAVEEFVHAGYDLDAAAILLCESDGTPEEV ADEIVRMTAVLREQGATRIQISRSESERLRFWSGRKNAFPAAGRISPDYYCMDGTVPRRS IGPLLARIEAMEKTYNLRCINVFHAGDGNMHPLILFNGNDLDEWHRAEAFGSDILEACVE LGGTVTGEHGVGIEKINSMCVQFSPEERDTFHAVKRAFDAPGLLNPDKGIPTRARCAEYG KMHVRGGLLPHPELPRF