Protein Info for BPHYT_RS03500 in Burkholderia phytofirmans PsJN

Annotation: glycolate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 PF13183: Fer4_8" amino acids 22 to 89 (68 residues), 46.1 bits, see alignment E=2.4e-15 PF13237: Fer4_10" amino acids 23 to 86 (64 residues), 29.1 bits, see alignment E=3.2e-10 PF13534: Fer4_17" amino acids 24 to 89 (66 residues), 40.9 bits, see alignment E=9.4e-14 PF12838: Fer4_7" amino acids 24 to 89 (66 residues), 42.1 bits, see alignment E=3.8e-14 PF02754: CCG" amino acids 171 to 254 (84 residues), 45.4 bits, see alignment E=2.8e-15 amino acids 304 to 387 (84 residues), 53.9 bits, see alignment E=6.4e-18

Best Hits

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 100% identity to bpy:Bphyt_0718)

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXA3 at UniProt or InterPro

Protein Sequence (408 amino acids)

>BPHYT_RS03500 glycolate oxidase (Burkholderia phytofirmans PsJN)
MQTNLADFIRNTPDGDEADGILRNCVHCGFCTATCPTYQILGDELDGPRGRIYLIKQMVE
GAPVTRSTQVHLDRCLTCRNCESTCPSGVQYGRLVEIGRKLTEEKVTRPLGQRLVRRMLA
SFVPNSALFTPTMRIGQHFRPLLPKKLRDKVPARQRPLEWPTAKHPRKMLMLAGCVQPSM
MPNVNIATARVFDALGVETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYIEQGV
EAIVMNASGCGATVKEYAHLLRHDPAYAEKARRVVELTRDVAEILPEFEEALVSVTRRRS
VHTVAFHPPCTLQHGQQIRGKVEHLLTALGVEVRLPADSHLCCGSAGTYSLTQPKLSYAL
RDQKLDRLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIELVEHMLSV