Protein Info for BPHYT_RS03425 in Burkholderia phytofirmans PsJN

Annotation: preprotein translocase subunit SecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 136 to 154 (19 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 188 to 210 (23 residues), see Phobius details amino acids 240 to 257 (18 residues), see Phobius details amino acids 263 to 292 (30 residues), see Phobius details PF07549: Sec_GG" amino acids 32 to 55 (24 residues), 28.9 bits, see alignment (E = 5.9e-11) TIGR00966: protein-export membrane protein SecF" amino acids 38 to 283 (246 residues), 254.5 bits, see alignment E=1.3e-79 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 95 to 280 (186 residues), 168.8 bits, see alignment E=1.4e-53 PF02355: SecD_SecF" amino acids 107 to 291 (185 residues), 213.5 bits, see alignment E=1.9e-67

Best Hits

Swiss-Prot: 40% identical to SECF_VIBAL: Protein translocase subunit SecF (secF) from Vibrio alginolyticus

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 100% identity to bpy:Bphyt_0702)

MetaCyc: 41% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SX87 at UniProt or InterPro

Protein Sequence (316 amino acids)

>BPHYT_RS03425 preprotein translocase subunit SecF (Burkholderia phytofirmans PsJN)
MEFFRFRKDIPFMQRALIFNAISLLTFLAAVFFLLHRGLHLSVEFTGGTVVEVQYPGAAP
LEPVRATLGKLGYADAQVQNFGTSRDVLIRLPLKQGYTSAQQSDQVMTALKAETPQVQLQ
RVEFVGPQVGKELATDGLLALACVVVGIVIYLSFRFEWKYAVAGVIANLHDVIIILGFFA
FFQWEFSLSVLAAVLAVLGYSVNESVVIFDRIRETFRRERKLTVIEVINHAITSTMSRTI
ITHGCTQMMVLSMFLFGGPTLHYFALALTVGILFGIYSSVFVAAALAMWFSVKREDLIKD
RKDRPDADDPNAGAQV