Protein Info for BPHYT_RS03400 in Burkholderia phytofirmans PsJN

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 PF17191: RecG_wedge" amino acids 130 to 283 (154 residues), 41.1 bits, see alignment E=3.6e-14 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 147 to 788 (642 residues), 707.7 bits, see alignment E=8e-217 PF04851: ResIII" amino acids 407 to 554 (148 residues), 33.4 bits, see alignment E=1.1e-11 PF00270: DEAD" amino acids 412 to 560 (149 residues), 85.9 bits, see alignment E=6.8e-28 PF00271: Helicase_C" amino acids 606 to 710 (105 residues), 58.4 bits, see alignment E=2e-19 PF19833: RecG_dom3_C" amino acids 739 to 801 (63 residues), 41 bits, see alignment 4.4e-14

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to bpy:Bphyt_0697)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SX82 at UniProt or InterPro

Protein Sequence (816 amino acids)

>BPHYT_RS03400 ATP-dependent DNA helicase RecG (Burkholderia phytofirmans PsJN)
MPLSDRRLPSVTDEVSAEPRRRVSRGKAAAQVSDAGLDVGAEVGFGAGKAARRGGESDGK
PVEEAGSESAKNAADTSGGERVDDLADRSGDTHAVEPDAIFGDRPAGKPGPKVAAKSAAK
SGAKSAAKPAEKTADKLAKLGLTRDIDLVLHLPMRYEDETSLTPIGHLLPGGISQTEGVV
FDNEIAYRPRRQLLVKLRDDAGDELVLRFLNFYGSQVKQMAIGARLRVRGDVRGGFFGME
MVHPAVRVVDEDTPLPQALTPVYPSTAGVTQAYLRKSIDNALSRTSLPELLPEAVARTFM
APLGVPSLMDAVRTLHHPGVQSDETALIDGTHPAWVRIKFEELLAQQMSLKRAHDERRTR
AAPAMPRRKLGDEAALVARLLKALPFSLTAAQERVGGEIALDLTQPHPMQRLLQGDVGSG
KTIVAALAAAQAIDAGYQAALMAPTEILAEQHARKLRGWLEPLGVSVAWLAGSLKTREKR
AAIEAAALGTAQLVIGTHAIIQDAVEFARLGLVIVDEQHRFGVAQRLALRAKAQNAADGA
RDFQPHQLMMSATPIPRTLAMTYYADLDVSTIDELPPGRTPILTKLVSDARREEVIGRVR
EAALTGRQVYWVCPLIEESETLQLQTAVETYETLVAALPELKVGLVHGRLAPAEKAAVMD
AFTRNEVQLLVATTVIEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGSAASVCVLL
YTGPLSMTARARLQTMRETTDGFEIARRDLEIRGPGEFLGARQSGAAMLRFADLQNDQWL
IEPAREAAAVLLEKYPEVVMQHLARWLGAREQFLKA