Protein Info for BPHYT_RS03355 in Burkholderia phytofirmans PsJN

Annotation: glutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 48 (19 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details PF01645: Glu_synthase" amino acids 153 to 470 (318 residues), 284.5 bits, see alignment E=6.9e-89

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0688)

Predicted SEED Role

"Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SX73 at UniProt or InterPro

Protein Sequence (536 amino acids)

>BPHYT_RS03355 glutamate synthase (Burkholderia phytofirmans PsJN)
MFSRRYLAMWCAAALFAVCAALADTHHISWFWIIVPLALVALGLFDLTQQRHAILRNYPL
WGHFRFLFEFIRPEIRQYFVEGDTDEKPFSRAQRSIVYQRAKNDVDSRPYGTELDVKATA
HEWISHSLAPTTLDNHDFRIVVGPDRAQPYSMSIFNVSAMSFGSLSANAIMALNLGAKKG
NFAHDTGEGSMSKYHREHGGDIIWEIASGYFGCRNDDGTFNAEKFAKQAAEPQVKMIEVK
LSQGAKPGHGGVLPAAKITPEIAETRGVPMGRDCISPATHSEFSTPRGLLEFVDRLRTLS
GGKPTGFKLCIGHPWEFFGIAKAMLETCILPDFIVVDGAEGGTGAAPLEFTDHVGVPLQE
GLLLVHNTLVGIGLRQRIRIGASGKMITAFDITKTLAIGADWVNAARGFMFAVGCIQAQT
CHTGRCPTGVATQDPVRQRALVVPDKADRVFNFHHNTLHALKEIIQAAGLKHPAELRAHH
IVRRVSSHEVQLMSELLKYLEPNDLLNGNYRYSLFEKYWPVAQSDSFSPKVELAAT