Protein Info for BPHYT_RS02760 in Burkholderia phytofirmans PsJN

Annotation: cytochrome BD oxidase subunit II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 transmembrane" amino acids 9 to 37 (29 residues), see Phobius details amino acids 72 to 98 (27 residues), see Phobius details amino acids 118 to 141 (24 residues), see Phobius details amino acids 164 to 187 (24 residues), see Phobius details amino acids 205 to 228 (24 residues), see Phobius details amino acids 261 to 282 (22 residues), see Phobius details amino acids 291 to 314 (24 residues), see Phobius details amino acids 334 to 356 (23 residues), see Phobius details TIGR00203: cytochrome d ubiquinol oxidase, subunit II" amino acids 1 to 378 (378 residues), 478.7 bits, see alignment E=7.2e-148 PF02322: Cyt_bd_oxida_II" amino acids 6 to 361 (356 residues), 347.1 bits, see alignment E=4.9e-108

Best Hits

Swiss-Prot: 52% identical to CYDB_ECOLI: Cytochrome bd-I ubiquinol oxidase subunit 2 (cydB) from Escherichia coli (strain K12)

KEGG orthology group: K00426, cytochrome bd-I oxidase subunit II [EC: 1.10.3.-] (inferred from 100% identity to bpy:Bphyt_0567)

MetaCyc: 52% identical to cytochrome bd-I subunit 2 (Escherichia coli K-12 substr. MG1655)
RXN0-5266 [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]

Predicted SEED Role

"Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Conserved gene cluster associated with Met-tRNA formyltransferase or Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXE7 at UniProt or InterPro

Protein Sequence (378 amino acids)

>BPHYT_RS02760 cytochrome BD oxidase subunit II (Burkholderia phytofirmans PsJN)
MDYATLKLIWWVLIGVLLIGFALTDGFDMGAAILLPFIGKTDAERRIVVNTVGATWEGNQ
VWLITAGGAMFAAWPLVYAASFSGFYFAMLLVLFALFFRPVGFDYRSKREDPRWRTAWDW
ALFVGGFVPALVFGVAFGNLLQGVPFSFDTDLRVTYHGGFFELLNPFAVLCGLVSVSMLA
AHGAAFVKMKADDIVAERASLALRIASFATVVLFLIAGALIATMIGGYQVIDAPPLDMVA
NPLMKNVIGAPGLWLTNYANYPWMVSAPVVGLVGGVLATLLARSRFEKSAFLSTSLMIIG
VILTAGFSMFPFIMPSSLDGRSSLTVWDSTSSRMTLQIMLIAVIIFLPIILIYTSWVYRV
MRGKVTAAALEENHHTMY