Protein Info for BPHYT_RS02745 in Burkholderia phytofirmans PsJN

Updated annotation (from data): N-acetylglucosamine-specific PTS system, IIBC components (nagE)
Rationale: Specifically important on NAG and cofit with the I-HPr-IIA component nearby (BPHYT_RS02740). CDD or PFam do not show any sign of this gene containing a IIA component, so the SEED annotation is misleading. 62% similar to H16_A0312 (nagE) and 58% similar to PA3761, which are both involved in NAG utilization (PMCid:PMC3127587, PMCid:PMC2542419). KEGG_correct.
Original annotation: PTS system N-acetylglucosamine-specific transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 72 to 89 (18 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 134 to 154 (21 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 194 to 215 (22 residues), see Phobius details amino acids 229 to 251 (23 residues), see Phobius details amino acids 260 to 278 (19 residues), see Phobius details amino acids 284 to 307 (24 residues), see Phobius details amino acids 311 to 331 (21 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details TIGR01998: PTS system, N-acetylglucosamine-specific IIBC component" amino acids 9 to 467 (459 residues), 604.8 bits, see alignment E=1.1e-185 PF02378: PTS_EIIC" amino acids 14 to 302 (289 residues), 249 bits, see alignment E=6.6e-78 TIGR00826: PTS system, glucose-like IIB component" amino acids 359 to 456 (98 residues), 60.5 bits, see alignment E=1.6e-20 PF00367: PTS_EIIB" amino acids 399 to 430 (32 residues), 43.6 bits, see alignment (E = 1.6e-15) amino acids 513 to 544 (32 residues), 29.7 bits, see alignment (E = 3.5e-11)

Best Hits

KEGG orthology group: K02803, PTS system, N-acetylglucosamine-specific IIB component [EC: 2.7.1.69] K02804, PTS system, N-acetylglucosamine-specific IIC component (inferred from 100% identity to bpy:Bphyt_0564)

Predicted SEED Role

"PTS system, N-acetylglucosamine-specific IIA component / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component" in subsystem Chitin and N-acetylglucosamine utilization or Sialic Acid Metabolism (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXE4 at UniProt or InterPro

Protein Sequence (591 amino acids)

>BPHYT_RS02745 N-acetylglucosamine-specific PTS system, IIBC components (nagE) (Burkholderia phytofirmans PsJN)
MDGNPFLKIQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
GVAVGFAKDNNGVAGLAGAIGYLIEVAVMKDINDKLNMGVLSGIVAGIVAGLLYNRYKDI
KLPDYLAFFGGKRFVPIVTGVVCLVLGIAFGYVWQPVQAVIDTAGHWLTTAGALGAFVFG
VLNRLLLVTGLHHILNSLTWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPVMM
FGLPAACLAMFHEAPKERRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHALL
TGISLAICSALGIHLGFTFSAGAIDYVLNYGLSTRGWWAIPIGLVYMVVYYGLFRFFIRK
FNMATPGREPAAADEQVDSFAAGGFVSPVAGTAVPRAQRYIAALGGASNLSVVDACTTRL
RLSVVDSNKVSENELKTIGARGVLKRGSTNVQVIIGPEADIIADEIRTVIAQGGGDAVKP
AAAAPAQVVAAAPVAASVAQGSGPLDPDPLRWLAVFGGAGNVLSLDAIAATRLRIVVRDP
SAVDRQRLATLDTAWISADTFHIVVGDAAQRYAEKLATRTTQSGGATPLPA