Protein Info for BPHYT_RS02740 in Burkholderia phytofirmans PsJN

Updated annotation (from data): N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF)
Rationale: Specifically important on NAG and cofit with the IIBC component nearby (BPHYT_RS02745). 58% identical to nagF or H16_A0311 of R. eutropha, which is important for NAG utilization (PMCid:PMC3127587). 58% similar to PA3760, which is required for NAG utilization in P. aeruginosa (PMCid:PMC2542419)
Original annotation: PTS glucose transporter subunit IIA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 PF00358: PTS_EIIA_1" amino acids 10 to 133 (124 residues), 146 bits, see alignment E=1.2e-46 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 10 to 130 (121 residues), 126.9 bits, see alignment E=6.4e-41 TIGR01003: phosphocarrier, HPr family" amino acids 178 to 255 (78 residues), 54.3 bits, see alignment E=1.6e-18 PF00381: PTS-HPr" amino acids 179 to 255 (77 residues), 62.5 bits, see alignment E=8e-21 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 294 to 844 (551 residues), 462.3 bits, see alignment E=3.1e-142 PF05524: PEP-utilisers_N" amino acids 295 to 414 (120 residues), 74.9 bits, see alignment E=1.6e-24 PF00391: PEP-utilizers" amino acids 440 to 512 (73 residues), 72.5 bits, see alignment E=4.4e-24 PF02896: PEP-utilizers_C" amino acids 540 to 823 (284 residues), 336.2 bits, see alignment E=3.9e-104

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to bpy:Bphyt_0563)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) / PTS system, glucose-specific IIA component" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXE3 at UniProt or InterPro

Protein Sequence (854 amino acids)

>BPHYT_RS02740 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (Burkholderia phytofirmans PsJN)
MSHSEGHIVLLAPMTGPVVPLANVPDPVFSGGMFGDGIGVDPLEGRLVAPCDATVTHLAR
TGHAVTLATAEGAEILLHIGIDTVELNGKGFAPMVAQGAHVRAGDVLIEFDQDQVALNAP
SLVSVIAIANSDAFEIVERVQGGLLKAGETPLLVLRARDGAAAEASRQLSSTNVTEEARQ
QVTLVHAGGLHARPAARAREAARGFDARVEVRYEGRKAAIESVVGLLGLGAGEGATVELL
GMGPQAAAAVAAIANELTREAHGEVEEKPARQSSPAPQAVARPAGETLAPNTLAGVCAAP
GVAVGKLVRWDDADIDPPEKANGTSAAESRLLDKAIATVDADLDTTVRDASQRGAVGEAG
IFSVHRVLLEDPTLLDAARDLISLGKSAGFAWREAIRAQIAILTNIEDALLAERAADLRD
IEKRVLRALGYTSATARTLPEEAVLAAEEFTPSDLSTLDRSRVTALVMARGGATSHAAIL
ARQAGIPALVAVGDALHAIPEGTQVVVNATTGRLEFAPTELDVERARLERTRLADVREAN
RRTSQQAAVTSDGRAIEVAANIATLDDAKTAVENGADSVGLLRTELLFIHRAAAPTTDEH
RQSYQAIVDALSGRTAIIRTLDVGADKEVDYLTLPPEPNPALGLRGIRLAQVRPDLLDDQ
LRGLLAVQPLGAVRILLPMVTDVGELIRIRKRIDEFARELGRTEPIEVGVMIEVPSAALL
ADQLAQHADFLSIGTNDLTQYTLAMDRCQADLAAQADGLHPAVLRLIAATVQGADKHGKW
VGVCGALAGDPLAMPLLVGLGVTELSVDPVSVPGIKARVRNLDYQLCRQRAQDALALESA
QAVRAASRETWPLD