Protein Info for BPHYT_RS02575 in Burkholderia phytofirmans PsJN

Annotation: phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 5 to 575 (571 residues), 538.5 bits, see alignment E=8.7e-166 PF05524: PEP-utilisers_N" amino acids 6 to 129 (124 residues), 106.9 bits, see alignment E=1.2e-34 PF00391: PEP-utilizers" amino acids 161 to 231 (71 residues), 82.4 bits, see alignment E=2.3e-27 PF02896: PEP-utilizers_C" amino acids 258 to 551 (294 residues), 375.1 bits, see alignment E=3.4e-116

Best Hits

Swiss-Prot: 69% identical to PT1_CUPNH: Phosphoenolpyruvate-protein phosphotransferase (phbI) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 100% identity to bpy:Bphyt_0531)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SX12 at UniProt or InterPro

Protein Sequence (582 amino acids)

>BPHYT_RS02575 phosphoenolpyruvate-protein phosphotransferase (Burkholderia phytofirmans PsJN)
MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIESEIERFRTAQQHVHYELETL
RADLAADAPSEMGAFINVHSMILNDAMLVQETFDLIRTRRYNVEWALTEQLERLSRHFDD
IEDEYLRERKADIEQVVERVLKALAGASVALADGVHGACDEMIVVAHDIAPADMMQFKTQ
TFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
VLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANIELPDDARAAIDAGATGVGL
FRTEFLFMNHKDRLPEEEEQFGAYRRAVELMNGLPVTIRTIDVGADKPLDSMSGGDGYET
AANPALGLRAIRWSLSEPQMFLTQLRAILRASAFGSVKILIPMLAHAQEIDQTLDLIREA
KRQLDDAGMAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNS
VAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPTMTRLLLGMGLTEFSMHPSQ
LLVVKQEVLRSHLKTLEKPVADVLASFEPEEVQAALKRVMLA