Protein Info for BPHYT_RS02550 in Burkholderia phytofirmans PsJN

Annotation: ammonia channel protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 105 to 127 (23 residues), see Phobius details amino acids 136 to 160 (25 residues), see Phobius details amino acids 205 to 225 (21 residues), see Phobius details amino acids 232 to 252 (21 residues), see Phobius details amino acids 265 to 288 (24 residues), see Phobius details amino acids 301 to 321 (21 residues), see Phobius details amino acids 333 to 353 (21 residues), see Phobius details amino acids 364 to 382 (19 residues), see Phobius details amino acids 388 to 408 (21 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details amino acids 452 to 472 (21 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 103 to 499 (397 residues), 442.6 bits, see alignment E=6.7e-137 PF00909: Ammonium_transp" amino acids 105 to 500 (396 residues), 397.7 bits, see alignment E=2.5e-123

Best Hits

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 100% identity to bpy:Bphyt_0526)

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SX07 at UniProt or InterPro

Protein Sequence (502 amino acids)

>BPHYT_RS02550 ammonia channel protein (Burkholderia phytofirmans PsJN)
MRKLMMSLLMAGSLLAGGIGAALADDASAPAAASAAAPDTTASAPAPDASAAPAAAAASA
PAADASAAPAASASAAAPAASDAAPAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIP
GLALFYGGMVRKKSVLAILMQSFAITCLVSIIWVVVGYTLAFTPGNSFIGGFSRFFMAGM
NYIKGDKATTLTVSHLAPTIPETVYVVYQMTFAIITPALITGAFADRMKFSAMLVFMTLW
SIIVYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRVGYGKEAMA
PHNLTLTLIGGAMLWVGWFGFNAGSAVAADGRAGFAMFATQVATAAAALAWMFAEWATKG
KPSVLGIVSGAVAGLVAITPASGFVGMAGSLVIGIAAGVICFWSATWLKHKLGYDDSLDA
FGVHCVGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMG
LRVTEEQEREGLDVTLHGEHVE