Protein Info for BPHYT_RS02290 in Burkholderia phytofirmans PsJN

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 PF03485: Arg_tRNA_synt_N" amino acids 12 to 102 (91 residues), 76.1 bits, see alignment E=4.2e-25 TIGR00456: arginine--tRNA ligase" amino acids 13 to 596 (584 residues), 404.6 bits, see alignment E=3.1e-125 PF00750: tRNA-synt_1d" amino acids 113 to 413 (301 residues), 66.8 bits, see alignment E=2.7e-22 PF05746: DALR_1" amino acids 473 to 596 (124 residues), 113.3 bits, see alignment E=1.1e-36

Best Hits

Swiss-Prot: 100% identical to SYR_PARPJ: Arginine--tRNA ligase (argS) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 100% identity to bpy:Bphyt_0473)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SWV4 at UniProt or InterPro

Protein Sequence (596 amino acids)

>BPHYT_RS02290 arginine--tRNA ligase (Burkholderia phytofirmans PsJN)
MLPAHKLTLETLLAETVKQVATASQGASEAAFVSPAITLERPKVAAHGDVACNVAMQLAK
PLRANPRQLAQQIVDALLAQPQAKGLVEAAEVAGPGFINLRLAAAAKQAVIAAVFAEKEA
FGRSQRDAGKHVLIEFVSANPTGPLHVGHGRQAALGDALSNVLASQGYDVHREFYYNDAG
VQIQTLALSTQARARGLAPGDAGWPASAYNGEYIAEIARDYLNGVTVAASDGEPVTGARD
VEDLEAIRRFAVAYLRREQDMDLQAFGVKFDQYYLESSLYKEGRVEKTVEALIAAGKTYE
QEGALWLRTTDDGDDKDRVMRKTDGTYTYFVPDVAYHVAKWERGFTKVINVQGSDHHGTI
ARVRAGLQGLGVGIPKGYPDYILHKMVTVMRNGEEVKISKRAGSYVTVRDLIEWSGGATP
GSEAAVDLIDEETIRRGRDAVRFFLISRKADTEFVFDIDLALKQNDENPVHYVQYAHARI
CSVIAECKARYSTDESTLADVDVSPLTSERAMALLNKLAEFPDMLQHAADELAPHAVAFY
LRDLAGEFHSFYNDRTERVLVDDAAERNARVALLAATRQVLANGLATIGVSAPVKM