Protein Info for BPHYT_RS02155 in Burkholderia phytofirmans PsJN

Annotation: dithiobiotin synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 PF13500: AAA_26" amino acids 11 to 218 (208 residues), 142.3 bits, see alignment E=1.8e-45 PF01656: CbiA" amino acids 12 to 216 (205 residues), 34.1 bits, see alignment E=2.4e-12 TIGR00347: dethiobiotin synthase" amino acids 12 to 182 (171 residues), 152.4 bits, see alignment E=6.2e-49

Best Hits

Swiss-Prot: 76% identical to BIOD_PARP8: ATP-dependent dethiobiotin synthetase BioD (bioD) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 100% identity to bpy:Bphyt_0445)

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SWS6 at UniProt or InterPro

Protein Sequence (247 amino acids)

>BPHYT_RS02155 dithiobiotin synthetase (Burkholderia phytofirmans PsJN)
MSGLNQPALSLFVTGTDTEIGKTFVSSALLRGFVREGLQAAAMKPIAAGAFELNSVLHNE
DADQLDAASNVLLPPAMRTPYLLKEPAAPHIAAALENVTFDLDHIVDCHAQALKRAEIVV
VEGVGGFRVPLTATQDTADLAVALKLPVVLVVGMRLGCISHALLTAEAIAARGLTLAGWV
ANRIDPDMTFPDENIASIREHLAREYDAPLLGIVPNLNPALPELAADQLDINRLLQTLRH
MQTHSQR