Protein Info for BPHYT_RS02145 in Burkholderia phytofirmans PsJN

Annotation: sulfate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 23 to 336 (314 residues), 435.2 bits, see alignment E=7.1e-135 PF01547: SBP_bac_1" amino acids 47 to 282 (236 residues), 47.4 bits, see alignment E=4.2e-16 PF13531: SBP_bac_11" amino acids 53 to 289 (237 residues), 142.9 bits, see alignment E=2.1e-45 PF13343: SBP_bac_6" amino acids 140 to 308 (169 residues), 29.1 bits, see alignment E=1e-10

Best Hits

Swiss-Prot: 56% identical to SUBI_SALTY: Sulfate-binding protein (sbp) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_0443)

MetaCyc: 55% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SWS4 at UniProt or InterPro

Protein Sequence (341 amino acids)

>BPHYT_RS02145 sulfate ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MAGKTGTSRWLAAGMTAITLALAGMSTAHADTSLLNVSYDVTRELYKDINAGFIAAYKQK
SGENVSIRQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLAEKGQLVPANWRARLPNDS
APYTTTMVFLVRKGNPKHIKDWDDLARPGVQVVIANPKTSGNGRYTYLAAWGYRKQHGGT
DAQALDFEKEIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGLGGGNFEAV
YPSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSSAAQEIIAQHHLRPRDKTVLAKH
AAEFKPIKTFTVEEMFGSWQKAQQTHFSDGGTFDQIIVDKK