Protein Info for BPHYT_RS02085 in Burkholderia phytofirmans PsJN

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02310: B12-binding
  • amino acids 22 to 122 (101 residues), 57.1 bits, 3.2e-19
• PF03308: MeaB
  • amino acids 287 to 526 (240 residues), 132 bits, E=4.4e-42
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 771 to 1297 (527 residues), 388.1 bits, E=5e-120
• PF01642: MM_CoA_mutase
  • amino acids 774 to 1291 (518 residues), 419.4 bits, E=4.1e-129

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See B2SWR2 at UniProt or InterPro

Protein Sequence (1298 amino acids)

>BPHYT_RS02085 methylmalonyl-CoA mutase (Burkholderia phytofirmans PsJN)
MTDLSTPQRAGSHKLPAGRRLRFVTAAALFDGHDASINIMRRILQASGVEVIHLGHNRSV
DEVATAALQEDADGVAVSSYQGGHNEYFRYLVDLLRARGGERIKVFGGGGGVIVPEEISE
LESYGVEKIYSPQDGQRLGLQGMIDDMIARCAEGARAAEAVGQSRVGEWVDEFSKRGLPR
FDSRGDAGGDEDAVRNPFSQASHVGCATSVGHVANAADTADAADTADTADAADTADAADT
ADTADTADTAIAANTANTANTANTANTANTANTANTSDPASSTFRRLAQLISAFEAGAVD
ASAREKLSAQAQAKATATPVLGITGTGGAGKSSLTDELIRRFRLDYGDALTIAVLAIDPS
RRKSGGALLGDRIRMNAIGDWGGGARVYMRSMATREASSEVSDSLPDALMLCKAAGFDLI
VVETSGIGQGNAAIVPFVDESLYVMTPEFGAASQLEKIDMLDFAGFVAINKFDRKGAPDA
LRDVAKQVQRNRADFAKPPEAMPVFGTIASRFNDDGVTALYRHVAEALRKHGLRSGGGRL
AAPEGLRFSSGRNAIVPPARVRYLADIAQTIHAYRERADAQARLARERWQLVEARRMLGE
AGTQAGAVTQSRASASGNSSGNPNANSDADSDADSDANANANANANANANANANANANAN
ANANANASANANSNPDPNSNKHAPANVTSADTPSPLQQLDTLIAQRTASLGERERALLDT
WPQTVAAYSGAEHIVRIRDREIRTALTVTTLSGSEVRKVSLPKFADHGEILRWLMLDNLP
GYFPFTAGVFPFRRENEDPTRMFAGEGDPQRTNRRFKLLSEGMPAKRLSTAFDSVTLYGE
EPDERPDIYGKVGNSGVSVATLDDMKTLYDGFDLCAPETSVSMTINGPAPTILAMFFNVA
IDQQIARMTQQQGRPLTHDELSATRRAALENVRGTVQADILKEDQGQNTCIFSTEFSLKV
MGDIQAYFVEHGVRNFYSVSISGYHIAEAGANPISQLAYTLANGFTYVEAYLARGMSIDD
FAPNLSFFFSNGMDPEYTVLGRVARRIWAVAMRERYGANERSQKLKYHVQTSGRSLHAQE
IDFNDIRTTLQALIAIYDNCNSLHTNAFDEAITTPTEDSVRRAVAIQLIINREWGLAKNQ
NPNQGSFVIEELTDLVEEAVLAEFDRLTERGGVLGAMETGYQRGRIQDESMLYEHRKHDG
SYPIVGVNTFLSAHPHEAPQPIALARSTDDEKQSQLQRLRAFQSQHRDAAPAALERLKRA
VIDDENVFAVLMDVVRVCSLGQITHALFEVGGQYRRNM