Protein Info for BPHYT_RS02050 in Burkholderia phytofirmans PsJN
Updated annotation (from data): D-mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for: D-Mannose; D-Sorbitol; D-Fructose. Often annotated as N-acylglucosamine 2-epimerase, but phenotype on mannose is consistent with annotation as mannose isomerase. Its role on sorbitol (which is probably oxidized to sorbose or fructose by BPHYT_RS16120) or fructose is unclear - this might be a polar effect on fructokinase (BPHYT_RS02045), as there is a stronger phenotype for insertions with the antibiotic resistance marker in the non-coding orientation
Original annotation: N-acylglucosamine 2-epimerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0424)Predicted SEED Role
"D-mannose isomerase (EC 5.3.1.7)" in subsystem Mannose Metabolism (EC 5.3.1.7)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.3.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2SWQ5 at UniProt or InterPro
Protein Sequence (427 amino acids)
>BPHYT_RS02050 D-mannose isomerase (EC 5.3.1.7) (Burkholderia phytofirmans PsJN) MTQSDPLHPANGAAAAPPVESFRSRDFLLSHVQDTLRFYAPNVFDPSGGFFHFFRDDGSV YDKTTRHLVSSCRYVFNYAMAYRQFGDPQHLEYARHGLRFLREAHWDAQHEGYDWEIEWR DGKKRTLDATRHCYGLAFVLLAYSHAAMAGIEEAKPMIGATFELMEHRFWDAAAGLYADE ASPDWRVSSYRGQNANMHTTEALLAAHEATRHLVYLDRAERVASNITLRQAKLSQGLVWE HFHADWSVDWHYNEEDSSNIFRPWGFQPGHQTEWAKLLLILERFRPLPWLLPRAIELFDA AMAHAWDEDHGGLYYGFGPDGTVCDHDKYFWVQAETFATAALLGKRTGNERFWDWYDEIW RYSWAHFVDHEYGAWYRILTCDNRKYSDEKSPAGKTDYHTMGACYEVLAHALPDGAAAAP ESAEQTK