Protein Info for BPHYT_RS01630 in Burkholderia phytofirmans PsJN

Annotation: ATP-dependent protease ATPase subunit HslU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 4 to 448 (445 residues), 678.1 bits, see alignment E=2.9e-208 PF07728: AAA_5" amino acids 52 to 88 (37 residues), 22.1 bits, see alignment 2.6e-08 PF00004: AAA" amino acids 53 to 99 (47 residues), 28.7 bits, see alignment 3.3e-10 amino acids 248 to 338 (91 residues), 31.2 bits, see alignment E=5.5e-11 PF07724: AAA_2" amino acids 189 to 334 (146 residues), 97.6 bits, see alignment E=1.7e-31

Best Hits

Swiss-Prot: 100% identical to HSLU_PARPJ: ATP-dependent protease ATPase subunit HslU (hslU) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 100% identity to bpy:Bphyt_0342)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1N8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>BPHYT_RS01630 ATP-dependent protease ATPase subunit HslU (Burkholderia phytofirmans PsJN)
MSTMTPAEIVSELDKHIIGQGRAKKAVAVALRNRWRRQQVEDPLRQEITPKNILMIGPTG
VGKTEIARRLAKLADAPFIKIEATKFTEVGYVGRDVDSIVRDLIEISVKQTRETEMRKVR
TKAEDQAEDRILDILLPSARPVGFGASSSAADTVDESSTTRQTFRKRLREGLLDDKEIEL
DVEQPQVGMDIMGPPGMEDMTEQIRSMFANIGGGKKTRRKLKVKEALKVLTDEEAGKMLN
DEEVKTKAVQNVEQNGIVFLDEIDKIASRNEAGGGEVSRQGVQRDLLPLVEGTTINTKYG
MVKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELDSLSVNDFESILVSTDASLVKQY
QALLATEDVHLEFADDGIRRLAEIAYSVNEKTENIGARRLYTVIEKLLEEVSFAAGNHSG
RTVQIDAAYVDRALNEVAEDEDLSRYVL