Protein Info for BPHYT_RS01605 in Burkholderia phytofirmans PsJN

Annotation: HAD family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 TIGR01993: pyrimidine 5'-nucleotidase" amino acids 19 to 202 (184 residues), 147.1 bits, see alignment E=5.1e-47 PF00702: Hydrolase" amino acids 21 to 186 (166 residues), 42.1 bits, see alignment E=1.3e-14 PF13419: HAD_2" amino acids 21 to 203 (183 residues), 43.9 bits, see alignment E=2.9e-15 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 102 to 203 (102 residues), 36.5 bits, see alignment E=5.4e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0337)

Predicted SEED Role

"Pyridoxal-5'-phosphate phosphatase (EC 3.1.3.74), Alphaproteobacterial type" (EC 3.1.3.74)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.74

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1N3 at UniProt or InterPro

Protein Sequence (267 amino acids)

>BPHYT_RS01605 HAD family hydrolase (Burkholderia phytofirmans PsJN)
MRTPTSRNRRPHIAGGRPVWLFDLDNTLHQASHAIFPAINEGMTQYIIDALQVDLDEANR
LRTGYTQRYGATLLGLMRHHPLDPNDFLKVVHTFPDLGSMIRHERGVARLVAALPGRKIV
LTNAPEAYARAVLAELRIERLFEQVIAIEHMRDRRRWRAKPDHAMLRKAMRDAHVSLKDV
ILVEDTRSHLKNYRRLGIRTVWITGHLPRKPHADGVPTRLPGTGRPHYVDRSIRSLKSLR
LSTRSVRLKRQQTGRQPCSRSTSLTKS