Protein Info for BPHYT_RS01430 in Burkholderia phytofirmans PsJN

Annotation: phosphoenolpyruvate carboxykinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 PF17297: PEPCK_N" amino acids 25 to 245 (221 residues), 353.2 bits, see alignment E=5.5e-110 PF00821: PEPCK_GTP" amino acids 249 to 614 (366 residues), 556 bits, see alignment E=3e-171

Best Hits

Swiss-Prot: 89% identical to PCKG_PARP8: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 100% identity to bpy:Bphyt_0304)

MetaCyc: 53% identical to cytosolic phosphoenolpyruvate carboxykinase (GTP) (Homo sapiens)
Phosphoenolpyruvate carboxykinase (GTP). [EC: 4.1.1.32]; 4.1.1.32 [EC: 4.1.1.32]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1K0 at UniProt or InterPro

Protein Sequence (618 amino acids)

>BPHYT_RS01430 phosphoenolpyruvate carboxykinase (Burkholderia phytofirmans PsJN)
MNHPSFEGQPTIEAPAWVKHRKLIDWVQRVAAKTKPDRIVWCDGSQEEYDRLCAQMVEAG
TLKKLNPAKRPNSYLAWSDPSDVARVEDRTFICSQRREDAGPTNNWIAPAEMRSTLDGLF
EGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNMRIMTRMGRKVYDVLGEDGEF
VPCVHSVGAPLAAGAKDVSWPCNETKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIG
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
WEGMTDDAPAHLIDWQGKDWTPASAKESGRKAAHPNARFTAPASQCPSIDPDWENPAGVA
IDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLKTGERLEQLNAKLPRIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRI
EGSAQGEEHAFGVSPHYEDIDWSGLNFSREQFEQVISVDDAAWRAELALHAELFDTLQQG
LPPALTEAKRALEQKLAA