Protein Info for BPHYT_RS01275 in Burkholderia phytofirmans PsJN
Annotation: DNA-directed DNA polymerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02346, DNA polymerase IV [EC: 2.7.7.7] (inferred from 100% identity to bpy:Bphyt_0274)Predicted SEED Role
"DNA polymerase IV (EC 2.7.7.7)" in subsystem DNA repair, bacterial (EC 2.7.7.7)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.7
Use Curated BLAST to search for 2.7.7.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T0Y1 at UniProt or InterPro
Protein Sequence (385 amino acids)
>BPHYT_RS01275 DNA-directed DNA polymerase (Burkholderia phytofirmans PsJN) MNDRTRRIAHLDMDAFYASVELLRYPELRGKPVVIGGGRNATPKILADGTRRFARLRDYV GRGVVTTSTYEARAFGVFSAMGMMKAAQLAPDAILLPTDFESYRRYSRLFKDAVGAFTDQ MEDRGIDEIYIDLTAVTGESRELGDRIKAAVRQATSLTCSIAIAPNKLLAKIGSELDKPD GLTVLTMADLESRIWPLAAKKINGIGPRATERLAGIGIATVGELAAADPGLLQEHFGRTY AEWFARVARGIDDRPIVVASEPKSMSRETTFERDMHVNRDRVTLTPALTRLCERVADDLQ RKGYGGRTVGLKIKFADFRIVTRDLSLPEAVTDAAAIRRAAGECLKRVPLDRRIRLIGVR IGSLERTSDSTSAVSPAQHELPFDR