Protein Info for BPHYT_RS01065 in Burkholderia phytofirmans PsJN

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00732: GMC_oxred_N" amino acids 235 to 347 (113 residues), 40.6 bits, see alignment E=2.7e-14 PF05199: GMC_oxred_C" amino acids 450 to 572 (123 residues), 61.8 bits, see alignment E=1.1e-20

Best Hits

Swiss-Prot: 54% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0227)

MetaCyc: 57% identical to D-gluconate dehydrogenase dehydrogenase subunit (Pseudomonas fluorescens)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T0T7 at UniProt or InterPro

Protein Sequence (592 amino acids)

>BPHYT_RS01065 GMC family oxidoreductase (Burkholderia phytofirmans PsJN)
MTKSMKPVDVVVIGLGWTGSILSMELASEGLEVVALERGHNRDTSPDYTYPKAVDELKYG
IRGELFRRLSLETVTVRHQVGDTAVPYRQYNAFLLPDNVGGAGVHWNGQLYRPSPEDMKF
RSHNVERYGAGFLPADMTIQDYDVTWNELEPYFDRFEYLNGTSGQAGNLRGKVLSGGNPF
EGPRSRAYPTPPVADQYAAKLFASAARELGYHPFPQPSANCSEPYTNFYGVRLGPCNLCG
FCERFGCFLYSKGAPQTTILPALMRKPNFELRTQSHVVKINVDSTGKRATGVTYIDGAGN
EVFQPADLVILSSFQINNVRLMLLSGIGRPYDPVTGKGVIGKNFAYQMMSSTSVFYDKDV
PINPFIAAGSGGSQIVDEFNSDHFDHGPHGFLGGGYIIGGQTGGRPIQQLAVPHGTPAWG
AQWKRAAKDHYLHTTNVVAHGSVMAYRDAYLDLDPTYRDSLGLPMLRMTFDWHDNEYRMT
RFVTDRALEIVAKMNPKSHSTLMRKPGDHFNVREYQTTHTAGGVITGADPQSSALNRYLQ
SWDVPNLFVMGASAFPQGIGYNPTGLVGALAYFAADAIRTQYLKRPGALVQA