Protein Info for BPHYT_RS00795 in Burkholderia phytofirmans PsJN

Annotation: conjugal transfer protein TraJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 transmembrane" amino acids 39 to 63 (25 residues), see Phobius details PF04335: VirB8" amino acids 20 to 230 (211 residues), 166.8 bits, see alignment E=3.4e-53

Best Hits

KEGG orthology group: K03203, type IV secretion system protein VirB8 (inferred from 100% identity to bpy:Bphyt_0167)

Predicted SEED Role

"Inner membrane protein forms channel for type IV secretion of T-DNA complex (VirB8)" in subsystem Type 4 secretion and conjugative transfer or pVir Plasmid of Campylobacter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T045 at UniProt or InterPro

Protein Sequence (252 amino acids)

>BPHYT_RS00795 conjugal transfer protein TraJ (Burkholderia phytofirmans PsJN)
MSSEHDGRDAGDSLGDYRRALDFEVSVTHLQERSERRAWHVALACVTVAILCAVALAVMV
PFYRVVPLPIEVDRLTGEAQVIDVLDSKQVPMRDIEDKHWVEVYVRSRERYDWGLLQMDY
DRVLDMSDERVARDYRSVYNGPDSLDRQLGANAQRRIRILSTTLVPDEPGRAVVHIERTT
WKNGIDNAEPPQRFVITLAFTYRPSVFTRERTAIENPFGFRVTAYSRDSEYTPAAPLPAS
LPTLAPASGGAR