Protein Info for BPHYT_RS00520 in Burkholderia phytofirmans PsJN

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 transmembrane" amino acids 193 to 211 (19 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 12 to 515 (504 residues), 641.5 bits, see alignment E=4.8e-197 PF04262: Glu_cys_ligase" amino acids 16 to 376 (361 residues), 525.5 bits, see alignment E=2.9e-162

Best Hits

Swiss-Prot: 64% identical to GSH1_PSEPW: Glutamate--cysteine ligase (gshA) from Pseudomonas putida (strain W619)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to bpy:Bphyt_0110)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZZ1 at UniProt or InterPro

Protein Sequence (532 amino acids)

>BPHYT_RS00520 glutamate--cysteine ligase (Burkholderia phytofirmans PsJN)
MYSAPLRPGLDVLRDHIHLFNACHRGVERECLRVTGDGHLSLSPHPGGLGAALTHPQITT
DYSESLLEFITPALADPVRTLESLDDLHRSTYANLGDEFLWSSSMPCALPAEEEIPIAMY
GTSNTGRLKHVYRKGLALRYGRTMQCIAGIHYNFSLPQAIWPLLGGGNRRERTDRDLKSW
VYMGLIRNFHRYNWLLLYLFGASPAVNADFLRGRRHELERLDGETLYLPHATSLRMSDLG
YQSHAQSSLAPCYDSLTGYIDALVMAMGTPYPLYGQIGTHRGAEWVQLNTNILQSENEFY
SNIRPKRVARAGERPVEALKDQGVEYVEVRSLDINPFLPLGIDESQAHFLDAFLLFCALR
ESPLLRDGEFAQGVANGLTVAREGRRPGLVLQRGAQSVYLKDWSNELLADIAPLAEMLDC
AKGGGSHRQALAAQSAKVNDPSLTPSSMMLATMSERGVSFNRFAMDQSRLHAESFRKRPL
PADRLMAFRALADASRAEQARLEKEQASGFDAFVGAFQGSLLSLSGDHATLI