Protein Info for BPHYT_RS00130 in Burkholderia phytofirmans PsJN

Annotation: ATPase P

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 transmembrane" amino acids 88 to 106 (19 residues), see Phobius details amino acids 112 to 130 (19 residues), see Phobius details amino acids 142 to 158 (17 residues), see Phobius details amino acids 164 to 182 (19 residues), see Phobius details amino acids 315 to 335 (21 residues), see Phobius details amino acids 346 to 372 (27 residues), see Phobius details amino acids 650 to 672 (23 residues), see Phobius details amino acids 678 to 699 (22 residues), see Phobius details PF00403: HMA" amino acids 5 to 60 (56 residues), 26.5 bits, see alignment 1e-09 TIGR01511: copper-translocating P-type ATPase" amino acids 126 to 698 (573 residues), 397.7 bits, see alignment E=2e-122 TIGR01525: heavy metal translocating P-type ATPase" amino acids 144 to 698 (555 residues), 586 bits, see alignment E=1.9e-179 TIGR01494: HAD ATPase, P-type, family IC" amino acids 167 to 674 (508 residues), 259.5 bits, see alignment E=9.4e-81 PF00122: E1-E2_ATPase" amino acids 195 to 376 (182 residues), 184.6 bits, see alignment E=1.9e-58 PF00702: Hydrolase" amino acids 396 to 609 (214 residues), 116.3 bits, see alignment E=3.5e-37

Best Hits

Swiss-Prot: 46% identical to CADA_STAAR: Probable cadmium-transporting ATPase (cadA) from Staphylococcus aureus (strain MRSA252)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to bpy:Bphyt_0026)

MetaCyc: 70% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ99 at UniProt or InterPro

Protein Sequence (701 amino acids)

>BPHYT_RS00130 ATPase P (Burkholderia phytofirmans PsJN)
MRTAIRIMQMDCPTEEALIRKKFSRMPHVRSMEFNLMQRVLTVVHAPEALDSILAALRSL
DFTPELADAGPNAASGASAAPRAPSKPWWPLALAGMAAAGSEAAGWLGAPVWLPAGLAIL
AILSCGLTTYKKGWLAIRNGNLNINALMSIAVTGALLLRQWPEAAMVMVLFTIAELIEAK
SLDRARNAIQGLMQLTPEQASVQQLDGSWRLVDLKAIALGAVVRVKPGERIALDGEIVAG
RSSVDQAPITGESLPVDKAVGDAVFAGTINQTGSFDYRVTAAASNTTLARIIHAVEEAQG
TKAPTQRFVDQFARVYTPIVFAVALAVAIVPPLLFDGSWQAWVYKALVMLVIACPCALVI
STPVTIVSGLAAAARKGILIKGGAYLEQGRKLTRLALDKTGTLTHGKPVQTEFEILAEID
ALRCRTLAASLSGRSDHPVSMAIATAAKADGLKHITVAAFEAIAGRGVRGEIDGVPYWLG
NHRLIEELGRCSASLEARLDALESQGKTVVMLVDAERVLALFAVADTVKETSRAAIAELQ
RLGVSTAMLTGDNPHTAAAIAQQVGIDEARGNQLPEDKLNAVAQWSDDGATVGMVGDGIN
DAPALARADIGFAMGAMGTDTAIETADVALMDDDLRKIPSFIRLSKATHAVLVQNITLAL
GIKSVFLVLTVIGLGTMWMAVFADVGASLLVVANGLRLLRK