Protein Info for BPHYT_RS00105 in Burkholderia phytofirmans PsJN

Annotation: ethanolamine ammonia lyase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 PF06751: EutB" amino acids 12 to 445 (434 residues), 750.9 bits, see alignment E=2.2e-230

Best Hits

Swiss-Prot: 53% identical to EUTB_ECOLI: Ethanolamine ammonia-lyase heavy chain (eutB) from Escherichia coli (strain K12)

KEGG orthology group: K03735, ethanolamine ammonia-lyase large subunit [EC: 4.3.1.7] (inferred from 100% identity to bpy:Bphyt_0021)

MetaCyc: 53% identical to ethanolamine ammonia-lyase subunit alpha (Escherichia coli K-12 substr. MG1655)
Ethanolamine ammonia-lyase. [EC: 4.3.1.7]

Predicted SEED Role

"Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.7

Use Curated BLAST to search for 4.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ94 at UniProt or InterPro

Protein Sequence (464 amino acids)

>BPHYT_RS00105 ethanolamine ammonia lyase large subunit (Burkholderia phytofirmans PsJN)
MSYTETIGTRTYRFADLKTLLAKASPQRSGDQLAGVAAASEEERVAAKMALAQVPLRTFL
NEALIPYESDEVTRLVIDDHSPQAFAEISHLTVGDFRNWLLSSTTDADALTRISAGLTPE
MVAAVSKLMRNQDLIAAARKRPVITRFRNTVGLPGHMSVRLQPNHPTDDVKGIAASMLDG
LMYGCGDAMIGINPASDNLAAITKLLLMIDDFRQRYQVPTQSCVLTHVTNTIAAIEKGAP
VDLVFQSIAGTEKANAGFGISLALLQEAYEAGLSLKRGTVGNNLMYFETGQGSALSADAH
FGVDQQTCEVRAYAVARRFNPFLVNTVVGFIGPEYLYDGKQITRAGLEDHFCGKLLGVPM
GCDICYTNHAEADQDDMDNLLTLLGVAGINFIMGIPGADDVMLNYQSTSFHDALYVRDVL
GLRRAPEFEEWLESMQITDARGALLSASTQQPLLEGARDWMGIA