Protein Info for BPHYT_RS00090 in Burkholderia phytofirmans PsJN

Annotation: reverse transcriptase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 PF00078: RVT_1" amino acids 155 to 369 (215 residues), 86.9 bits, see alignment E=7.9e-29

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0018)

Predicted SEED Role

"Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)" (EC 2.7.7.49)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ91 at UniProt or InterPro

Protein Sequence (451 amino acids)

>BPHYT_RS00090 reverse transcriptase (Burkholderia phytofirmans PsJN)
MSLSSLQHVTYAIADAMLAGSPEPGGMVERMTLVLEESARWMDGLARHVAKRFAARWDSV
GRKEVSTVVAGMPGFVSAWRSEQPPRVVRVLTRPPFQRPPPPWLHDVALPQLPTLGDLAA
WLDIEPGDLGWFADRWRVPTRGAATPLHHYAYKAIEKRDGRCRIIEIPKPRLRALQRKVL
SGLLDRIPAHESVHGFRHGRNIVTFAAPHVGKAVVMRFDLTDFFASVHAGRVYSAFYALG
YPQAVARALTALCTNRIPSGRLLAPDVRERIDWRERQRYRNRHLPQGAPTSPALANLCAF
RLDLRLAGLARSVGATYTRYADDLAFSGDEELARMADRLCIRVAAIALEEGFGVNLRKTR
VMRRSARQHLAGVVVNSHANVARPEFDALKAVLTNCVRHGWRSQNRDDLADFRAHLAGRV
AHVAMVNAVRGARLRAVFERIEWEEEKPLDA