Protein Info for BBR_RS20420 in Bifidobacterium breve UCC2003

Annotation: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 TIGR00755: ribosomal RNA small subunit methyltransferase A" amino acids 27 to 292 (266 residues), 239 bits, see alignment E=2.6e-75 PF00398: RrnaAD" amino acids 30 to 289 (260 residues), 157.1 bits, see alignment E=2.5e-50

Best Hits

Swiss-Prot: 86% identical to RSMA_BIFLO: Ribosomal RNA small subunit methyltransferase A (rsmA) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K02528, 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase [EC: 2.1.1.182] (inferred from 86% identity to blo:BL0657)

Predicted SEED Role

"SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)" (EC 2.1.1.182)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.182

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (309 amino acids)

>BBR_RS20420 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA (Bifidobacterium breve UCC2003)
MSNNTSTTGHLLGAADIRRIAAEAGISPTKKFGQNFVIDPGTVRRIVREADVTTNDHVME
VGPGLGSLTLAILETGATMTAVEIDPPLAERLPRTVAEFMPEAADRLTVVNRDALTVTPD
NVPDFNGDTSFTLVANLPYNVATPILLTLLERFDSLGSFLVMVQKEVADRLAAKPGSKIY
GTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYAADDTQAPSHGKSSADRELV
FRLIDAAFGQRRKTLHAALKTIVPAEAFAAAGIDPTRRGETLTVTEFAALAAAVQSTSHA
TPTTDEVEQ